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One-pot synthesis and biochemical characterization of the novel magnetic phospholipase D nanoflowers modified with polyphenols
Molecular Catalysis ( IF 3.9 ) Pub Date : 2024-03-26 , DOI: 10.1016/j.mcat.2024.114056
Yue Zhang , Changhao Liu , Siqi Chen , Chengcheng Fan , Zhaoxia Jin , Fang Yu

Phospholipase D (PLD) can catalyze the hydrolytic cleavage of phosphodiester bonds and transphosphatidylation and has been widely employed in the chemical and pharmaceutical fields. To improve the stability and reusability of PLD, an immobilized enzyme system was developed using magnetic nanoparticles modified with polyphenols (dopamine and tannic acid) and metal hybrid nanoflowers to co-immobilize phospholipase D (PLD). This immobilization technique aimed to improve the enzymatic performance through increased surface area, reduced mass transfer limitations, and facilitated reuse. The hydrolytic and transphosphatidylation activities of PLD immobilized on polyphenol-modified magnetic nanoflowers (PDA-MNFs and TA-MNFs) were evaluated and compared to free PLD. PDA-MNFs and TA-MNFs showed significantly enhanced hydrolytic activity (19.9-fold and 21.7-fold increases, respectively) and transphosphatidylation activity (over 1.79-fold and 1.96-fold increases, respectively) compared to free PLD. Temperature and pH stability were also markedly improved for the immobilized PLD. After 10 reuse cycles, PDA-MNFs and TA-MNFs retained over 65 % of their initial transphosphatidylation activity. Therefore, this co-deposition and polymerization immobilization strategy using polyphenol-modified magnetic nanoflowers has the potential for efficiently increasing enzymatic performance and synthesizing industrially relevant compounds like phosphatidic acid and phosphatidylserine

中文翻译:


多酚修饰新型磁性磷脂酶D纳米花的一锅法合成及生化表征



磷脂酶D(PLD)可以催化磷酸二酯键的水解断裂和转磷脂酰化,已广泛应用于化学和制药领域。为了提高PLD的稳定性和可重复使用性,利用多酚(多巴胺和单宁酸)修饰的磁性纳米粒子和金属杂化纳米花来共同固定磷脂酶D(PLD),开发了固定化酶系统。这种固定技术旨在通过增加表面积、减少传质限制和促进重复使用来提高酶性能。评估了固定在多酚修饰的磁性纳米花(PDA-MNF 和 TA-MNF)上的 PLD 的水解和转磷脂酰化活性,并与游离 PLD 进行比较。与游离 PLD 相比,PDA-MNF 和 TA-MNF 显示出显着增强的水解活性(分别增加 19.9 倍和 21.7 倍)和转磷脂酰化活性(分别增加 1.79 倍和 1.96 倍)。固定化 PLD 的温度和 pH 稳定性也得到显着改善。经过 10 次重复使用循环后,PDA-MNF 和 TA-MNF 保留了超过 65% 的初始转磷脂酰化活性。因此,这种使用多酚修饰的磁性纳米花的共沉积和聚合固定策略具有有效提高酶性能并合成工业相关化合物(如磷脂酸和磷脂酰丝氨酸)的潜力
更新日期:2024-03-26
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