Juvenile hormone binding protein (JHBP) is a key regulator of JH signaling, and crosstalk between JH and 20-hydroxyecdysone (20E) can activate and fine-tune the mitogen-activated protein kinase cascade, leading to resistance to insecticidal proteins from Bacillis thuringiensis (Bt). However, the involvement of JHBP in the Bt Cry1Ac resistance of Plutella xylostella remains unclear. Here, we cloned a full-length cDNA encoding JHBP, and quantitative real-time PCR (qPCR) analysis showed that the expression of the PxJHBP gene in the midgut of the Cry1Ac-susceptible strain was significantly higher than that of the Cry1Ac-resistant strain. Furthermore, CRISPR/Cas9-mediated knockout of the PxJHBP gene significantly increased Cry1Ac susceptibility, resulting in a significantly shorter lifespan and reduced fertility. These results demonstrate that PxJHBP plays a critical role in the resistance to Cry1Ac protoxin and in the regulation of physiological metabolic processes associated with reproduction in adult females, providing valuable insights to improve management strategies of P. xylostella.

中文翻译：

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CRISPR/Cas9 介导的 PxJHBP 基因敲除导致小菜蛾对 Bt Cry1Ac 原毒素的敏感性增加，并降低寿命和产卵率


保幼激素结合蛋白 (JHBP) 是 JH 信号传导的关键调节因子，JH 和 20-羟基蜕皮激素 (20E) 之间的串扰可以激活和微调丝裂原激活蛋白激酶级联，从而导致苏云金杆菌对杀虫蛋白产生抗性。 BT）。然而，JHBP 在小菜蛾Bt Cry1Ac 抗性中的作用仍不清楚。在此，我们克隆了编码JHBP的全长cDNA，实时定量PCR（qPCR）分析表明，Cry1Ac敏感株中肠中PxJHBP基因的表达量显着高于Cry1Ac抗性株。此外，CRISPR/Cas9介导的PxJHBP基因敲除显着增加了Cry1Ac敏感性，导致寿命显着缩短并降低了生育能力。这些结果表明， PxJHBP在 Cry1Ac 原毒素抗性和成年雌性生殖相关生理代谢过程的调节中发挥着关键作用，为改善小菜蛾的管理策略提供了宝贵的见解。