Discovery of sesquiterpenoids from an actinomycete Crossiella cryophila through genome mining and heterologous expression,Bioorganic Chemistry

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Discovery of sesquiterpenoids from an actinomycete Crossiella cryophila through genome mining and heterologous expression
Bioorganic Chemistry ( IF 4.5 ) Pub Date : 2024-03-22 , DOI: 10.1016/j.bioorg.2024.107308
Qian Yang ₁ , Jupeng Tian ₁ , Shungen Chen ₁ , Ziyi Yang ₁ , Zengyuan Wang ₁ , Hui-Min Xu ₂ , Liao-Bin Dong ₁

Affiliation

Genome mining of the Actinomycete facilitated the discovery of a minimal terpenoid biosynthetic gene cluster of consisting of a class I terpene cyclase CryA and a CYP450 monooxygenase CryB. Heterologous expression of allowed the isolation and characterization of two new sesquiterpenoids, -viridiflorol () and cryophilain (). Notably, cryophilain () possesses a 5/7/3-fused tricyclic skeleton bearing a distinctive bridgehead hydroxy group. The combined in vivo and in vitro experiments revealed that CryA, the first -viridiflorol terpene cyclase, catalyzes farnesyl diphosphate to form the 5/7/3 sesquiterpene core scaffold and P450 CryB serves as a tailoring enzyme responsible for installing a hydroxy group at the bridgehead carbon.

中文翻译：

通过基因组挖掘和异源表达从放线菌 Crossiella Cryophila 中发现倍半萜类化合物

放线菌的基因组挖掘促进了由 I 类萜烯环化酶 CryA 和 CYP450 单加氧酶 CryB 组成的最小萜类生物合成基因簇的发现。的异源表达允许分离和表征两种新的倍半萜类化合物，-viridiflorol ()和cryophilin ()。值得注意的是，cryophilin () 具有 5/7/3-稠合三环骨架，带有独特的桥头羟基。体内和体外联合实验表明，CryA是第一个β-viridiflorol萜烯环化酶，催化法尼基二磷酸形成5/7/3倍半萜核心支架，而P450 CryB作为剪裁酶负责在桥头安装羟基。碳。

更新日期：2024-03-22

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