<br>静磁场增强颅骨缺损大鼠模型中人脱矿骨基质的骨重塑能力,Journal of Materials Chemistry B

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静磁场增强颅骨缺损大鼠模型中人脱矿骨基质的骨重塑能力
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2024-03-27 , DOI: 10.1039/d3tb02299d
SeyedJamal Hosseini _{1,

2} , Houman Parsaei ₃ , MirJavad Moosavifar _{1,

2,

4} , Narjes Tavakoli ₅ , Reza Ahadi ₆ , Kaveh Roshanbinfar ₇

Affiliation

超过2厘米的骨缺损的再生对人体来说是一个挑战，需要介入治疗。源自生物组织的脱矿骨基质（DBM）已用于骨再生，并具有显着的骨诱导和骨传导特性。然而，它们再生严重损伤的效率是有限的，因此需要额外的信号提示。由于骨骼的压电特性，外部物理刺激可以加速组织愈合。我们将人类 DBM 植入大鼠动物模型的临界尺寸颅骨缺损中，并将其暴露于外部磁场 (1 T) 以增强内源性骨形成。我们的体外实验表明，与细胞培养板相比，DBM 具有优异的细胞相容性。此外，14天后的碱性磷酸酶活性和28天的茜素红染色表明大鼠骨髓间充质干细胞在DBM上分化为骨谱系。计算机断层扫描图像与组织学分析表明，在受伤大鼠体内植入 DBM 显着增强了骨再生。值得注意的是，与单独的 DBM 移植相比，将 DBM 移植与每天 2 小时暴露于 1 T 磁场持续 2 周（术后第 7 至 21 天）相结合可显着改善骨再生。这项研究表明，利用外部磁刺激可显着增强同种异体骨移植再生临界尺寸骨缺损的潜力。

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Static magnetic field enhances the bone remodelling capacity of human demineralized bone matrix in a rat animal model of cranial bone defects

The regeneration of bone defects that exceed 2 cm is a challenge for the human body, necessitating interventional therapies. Demineralized bone matrices (DBM) derived from biological tissues have been employed for bone regeneration and possess notable osteoinductive and osteoconductive characteristics. Nevertheless, their efficiency in regenerating critically sized injuries is limited, and therefore additional signaling cues are required. Thanks to the piezoelectric properties of the bone, external physical stimulation is shown to accelerate tissue healing. We have implanted human DBM in critically sized cranial bone defects in rat animal models and exposed them to an external magnetic field (1 T) to enhance endogenous bone formation. Our in vitro experiments showed the superior cytocompatibility of DBM compared to cell culture plates. Furthermore, alkaline phosphatase activity after 14 days and Alizarin red staining at 28 days demonstrated differentiation of rat bone marrow mesenchymal stem cells into bone lineage on DBM. Computer tomography images together with histological analyses showed that implanting DBM in the injured rats significantly enhanced bone regeneration. Notably, combining DBM transplantation with a 2 h daily exposure to a 1 T magnetic field for 2 weeks (day 7 to 21 post-surgery) significantly improved bone regeneration compared to DBM transplantation alone. This research indicates that utilizing external magnetic stimulation significantly enhances the potential of bone allografts to regenerate critically sized bone defects.

更新日期：2024-03-28

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