当前位置:
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Antifungal Activity and Putative Mechanism of HWY-289, a Semisynthetic Protoberberine Derivative, against Botrytis cinerea
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-03-27 , DOI: 10.1021/acs.jafc.3c08858
Jun-Xia An 1, 2 , Bao-Qi Zhang 1 , Hong-Jie Liang 1 , Zhi-Jun Zhang 1 , Ying-Qian Liu 1, 2, 3 , Shao-Yong Zhang 3
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-03-27 , DOI: 10.1021/acs.jafc.3c08858
Jun-Xia An 1, 2 , Bao-Qi Zhang 1 , Hong-Jie Liang 1 , Zhi-Jun Zhang 1 , Ying-Qian Liu 1, 2, 3 , Shao-Yong Zhang 3
Affiliation
![]() |
The emergence of resistant pathogens has increased the demand for alternative fungicides. The use of natural products as chemical scaffolds is a potential method for developing fungicides. HWY-289, a semisynthetic protoberberine derivative, demonstrated broad-spectrum and potent activities against phytopathogenic fungi, particularly Botrytis cinerea (with EC50 values of 1.34 μg/mL). SEM and TEM imaging indicated that HWY-289 altered the morphology of the mycelium and the internal structure of cells. Transcriptomics revealed that it could break down cellular walls through amino acid sugar and nucleotide sugar metabolism. In addition, it substantially decreased chitinase activity and chitin synthase gene (BcCHSV) expression by 53.03 and 82.18% at 1.5 μg/mL, respectively. Moreover, this impacted the permeability and integrity of cell membranes. Finally, HWY-289 also hindered energy metabolism, resulting in a significant reduction of ATP content, ATPase activities, and key enzyme activities in the TCA cycle. Therefore, HWY-289 may be a potential candidate for the development of plant fungicides.
中文翻译:
半合成原小檗碱衍生物 HWY-289 的抗灰霉病活性及推测机制
耐药病原体的出现增加了对替代杀菌剂的需求。使用天然产物作为化学支架是开发杀菌剂的潜在方法。 HWY-289 是一种半合成原小檗碱衍生物,对植物病原真菌,特别是灰葡萄孢 (Botrytis cinerea ) 表现出广谱和有效的活性(EC 50值为 1.34 μg/mL)。 SEM 和 TEM 成像表明 HWY-289 改变了菌丝体的形态和细胞的内部结构。转录组学表明,它可以通过氨基酸糖和核苷酸糖代谢来分解细胞壁。此外,在 1.5 μg/mL 浓度下,几丁质酶活性和几丁质合酶基因 ( BcCHSV ) 表达分别显着降低 53.03% 和 82.18%。此外,这影响了细胞膜的渗透性和完整性。最后,HWY-289还阻碍能量代谢,导致ATP含量、ATP酶活性和TCA循环中关键酶活性显着降低。因此,HWY-289可能是开发植物杀菌剂的潜在候选者。
更新日期:2024-03-27
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
半合成原小檗碱衍生物 HWY-289 的抗灰霉病活性及推测机制
耐药病原体的出现增加了对替代杀菌剂的需求。使用天然产物作为化学支架是开发杀菌剂的潜在方法。 HWY-289 是一种半合成原小檗碱衍生物,对植物病原真菌,特别是灰葡萄孢 (Botrytis cinerea ) 表现出广谱和有效的活性(EC 50值为 1.34 μg/mL)。 SEM 和 TEM 成像表明 HWY-289 改变了菌丝体的形态和细胞的内部结构。转录组学表明,它可以通过氨基酸糖和核苷酸糖代谢来分解细胞壁。此外,在 1.5 μg/mL 浓度下,几丁质酶活性和几丁质合酶基因 ( BcCHSV ) 表达分别显着降低 53.03% 和 82.18%。此外,这影响了细胞膜的渗透性和完整性。最后,HWY-289还阻碍能量代谢,导致ATP含量、ATP酶活性和TCA循环中关键酶活性显着降低。因此,HWY-289可能是开发植物杀菌剂的潜在候选者。