In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of such microscopes. Here, we introduce the next-generation mesoSPIM (“Benchtop”) with a significantly increased field of view, improved resolution, higher throughput, more affordable cost, and simpler assembly compared to the original version. We develop an optical method for testing detection objectives that enables us to select objectives optimal for light-sheet imaging with large-sensor cameras. The improved mesoSPIM achieves high spatial resolution (1.5 µm laterally, 3.3 µm axially) across the entire field of view, magnification up to 20×, and supports sample sizes ranging from sub-mm up to several centimeters while being compatible with multiple clearing techniques. The microscope serves a broad range of applications in neuroscience, developmental biology, pathology, and even physics.

2015 年，我们启动了 mesoSPIM 计划，这是一个开源项目，旨在使大型透明组织的光片显微镜检查变得更容易实现。与此同时，以更高的速度和分辨率对更大的样品进行成像的需求不断增加，需要对此类显微镜的功能进行重大改进。在这里，我们推出下一代 mesoSPIM（“Benchtop”），与原始版本相比，其视野显着增加，分辨率提高，吞吐量更高，成本更实惠，组装更简单。我们开发了一种用于测试检测物镜的光学方法，使我们能够选择最适合使用大传感器相机进行光片成像的物镜。改进的 mesoSPIM 在整个视场中实现了高空间分辨率（横向 1.5 µm，轴向 3.3 µm），放大倍数高达 20 倍，支持从亚毫米到几厘米的样本尺寸，同时与多种透明技术兼容。显微镜在神经科学、发育生物学、病理学甚至物理学领域有着广泛的应用。