Chitosan-Stabilized PtAu Nanoparticles with Multienzyme-Like Activity for Mixed Bacteria Infection Wound Healing and Insights into Its Antibacterial Mechanism,Small Structures

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Chitosan-Stabilized PtAu Nanoparticles with Multienzyme-Like Activity for Mixed Bacteria Infection Wound Healing and Insights into Its Antibacterial Mechanism
Small Structures ( IF 13.9 ) Pub Date : 2024-03-25 , DOI: 10.1002/sstr.202300553
Yujuan Wen ₁ , Wenxin Chen ₁ , Ruimei Wu ₁ , Jianpeng Guo ₂ , Xin Liu ₁ , Boling Shi ₁ , Cunshan Zhang ₁ , Lina Wu ₃ , Yanjie Zheng ₁ , Ailin Liu ₁ , Liqing Lin ₁

Affiliation

Traditional antibacterial agents are often observed to be ineffective because bacteria evolved to strains with greater antibiotic resistance. Here, vigorous chitosan-stabilized PtAu nanoparticles (CSPA) with multienzyme-like activity are successfully fabricated, which serve an effective artificial nanozyme to enhance antibacterial activity for mixed bacterial infection wound treatment. Ultrasmall size CSPA exhibits excellent hydrophilicity and biocompatibility, possesses strong oxidase- and peroxidase-like activity generating a substantial amount of ROS (

O_{2}^{-} $$ {\mathrm{O}}_{2}^{-}$$

, ¹O₂, ·OH) to cause oxidative damage to bacteria, also demonstrates nicotinamide adenine dinucleotide dehydrogenase-like activity disrupting the bacterial respiratory chains, and subsequently impedes adenosine triphosphate production. CSPA exhibits favorable broad-spectrum antibacterial activity at very low concentrations, prevents bacterial resistance, and completely inhibits bacterial biofilm formation. Antibacterial Mechanism of CSPA by the transcriptomics is further revealed that CSPA can induce bacterial oxidative stress, hinder bacterial energy metabolism, and disrupt the synthesis and function of bacterial cell walls and cell membranes. In vivo, CSPA inhibits the mixed bacterial population at the wound site and promotes wound healing in rats. This study introduces a novel antibacterial approach, providing important insight into the antibacterial mechanism of CSPA nanozymes and promoting the advancement of nanocatalytic materials in biomedical applications.

中文翻译：

具有类多酶活性的壳聚糖稳定的 PtAu 纳米粒子用于混合细菌感染伤口愈合及其抗菌机制

通常观察到传统抗菌剂无效，因为细菌进化成具有更强抗生素耐药性的菌株。在这里，成功制备了具有多酶活性的活性壳聚糖稳定的 PtAu 纳米颗粒（CSPA），它可以作为有效的人工纳米酶来增强混合细菌感染伤口治疗的抗菌活性。超小尺寸CSPA表现出优异的亲水性和生物相容性，具有强氧化酶和过氧化物酶样活性，产生大量ROS（

O_{2}^{-} $$ {\mathrm{O}}_{2}^{-}$$

， ¹ O ₂ ，· OH）对细菌造成氧化损伤，还表现出烟酰胺腺嘌呤二核苷酸脱氢酶样活性，可破坏细菌呼吸链，并随后阻碍三磷酸腺苷的产生。 CSPA在极低浓度下表现出良好的广谱抗菌活性，防止细菌耐药性，并完全抑制细菌生物膜的形成。转录组学进一步揭示CSPA的抗菌机制，CSPA可诱导细菌氧化应激，阻碍细菌能量代谢，破坏细菌细胞壁和细胞膜的合成和功能。在体内，CSPA 抑制大鼠伤口部位的混合细菌群并促进伤口愈合。本研究介绍了一种新颖的抗菌方法，为CSPA纳米酶的抗菌机制提供了重要的见解，并促进了纳米催化材料在生物医学应用中的进步。

更新日期：2024-03-25

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