Integrated physio-biochemical and transcriptomic analysis reveals the joint toxicity mechanisms of two typical antidepressants fluoxetine and sertraline on Microcystis aeruginosa,Science of the Total Environment

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Integrated physio-biochemical and transcriptomic analysis reveals the joint toxicity mechanisms of two typical antidepressants fluoxetine and sertraline on Microcystis aeruginosa
Science of the Total Environment ( IF 8.2 ) Pub Date : 2024-03-19 , DOI: 10.1016/j.scitotenv.2024.171802
Zhengxin Xie ₁ , Yunfan Nie ₁ , Mingyue Dong ₁ , Meng Nie ₁ , Jun Tang ₁

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Selective serotonin reuptake inhibitor (SSRI) antidepressants are of increasing concern worldwide due to their ubiquitous occurrence and detrimental effects on aquatic organisms. However, little is known regarding their effects on the dominant bloom-forming cyanobacterium, Microcystis aeruginosa. Here, we investigated the individual and joint effects of two typical SSRIs fluoxetine (FLX) and sertraline (SER) on M. aeruginosa at physio-biochemical and molecular levels. Results showed that FLX and SER had strong growth inhibitory effects on M. aeruginosa with the 96-h median effect concentrations (EC50s) of 362 and 225 μg/L, respectively. Besides, the mixtures showed an additive effect on microalgal growth. Meanwhile, both individual SSRIs and their mixtures can inhibit photosynthetic pigment synthesis, cause oxidative damage, destroy cell membrane, and promote microcystin−leucine−arginine (MC-LR) synthesis and release. Moreover, the mixtures enhanced the damage to photosynthesis, antioxidant system, and cell membrane and facilitated MC-LR synthesis and release compared to individuals. Furthermore, transcriptomic analysis revealed that the dysregulation of the key genes related to transport, photosystem, protein synthesis, and non-ribosomal peptide structures was the fundamental molecular mechanism underlying the physio-biochemical responses of M. aeruginosa. These findings provide a better understanding of the toxicity mechanisms of SSRIs to microalgae and their risks to aquatic ecosystems.

中文翻译：

综合理化和转录组学分析揭示了两种典型抗抑郁药氟西汀和舍曲林对铜绿微囊藻的联合毒性机制

选择性血清素再摄取抑制剂（SSRI）抗抑郁药因其无处不在的出现和对水生生物的有害影响而在世界范围内越来越受到关注。然而，关于它们对主要形成水华的蓝藻 Microcystis aeruginosa 的影响知之甚少。在这里，我们研究了两种典型的 SSRI 氟西汀（FLX）和舍曲林（SER）在生理生化和分子水平上对铜绿分枝杆菌的单独和联合影响。结果显示，FLX 和 SER 对铜绿分枝杆菌具有较强的生长抑制作用，96 h 中位效应浓度（EC50s）分别为 362 和 225 μg/L。此外，混合物对微藻生长显示出加性作用。同时，单个 SSRIs 及其混合物都可以抑制光合色素合成，引起氧化损伤，破坏细胞膜，并促进微囊藻毒素-亮氨酸-精氨酸（MC-LR）的合成和释放。此外，与个体相比，混合物增强了对光合作用、抗氧化系统和细胞膜的损伤，并促进了 MC-LR 的合成和释放。此外，转录组学分析显示，与运输、光系统、蛋白质合成和非核糖体肽结构相关的关键基因失调是铜绿分枝杆菌生理生化反应的基本分子机制。这些发现有助于更好地了解 SSRIs 对微藻的毒性机制及其对水生生态系统的风险。

更新日期：2024-03-19

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