Quinone Methide Based Self-Immobilizing Molecular Fluorescent Probes for In Situ Imaging of Enzymes,Chemistry - An Asian Journal

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Quinone Methide Based Self-Immobilizing Molecular Fluorescent Probes for In Situ Imaging of Enzymes
Chemistry - An Asian Journal ( IF 3.5 ) Pub Date : 2024-03-21 , DOI: 10.1002/asia.202400189
Yeru Miao ₁ , Zhen-Qing Yu ₁ , Shuai Xu ₁ , Mei Yan ₁

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Enzymes play important roles not only in normal physiological processes but in the development of many diseases. In situ imaging of enzymes with high-resolution in living systems would helpful for clinical diagnosis and treatment. However, many molecular fluorescent probes suffer from the drawback of diffusing away from the reaction site of enzymes even out of the cells, losing the in situ information and resulting in poor imaging resolution. Quinone methide (QM) based self-immobilizing probes allow the fluorescent signal to be immobilized near the target for an extended period without deactivating the target enzymes, ensuring that it will provide amplified signals and in situ information of the target with high resolution. In this review, we summarized the recent progress of QM-based self-immobilizing probes including their design strategies, working mechanisms, classifications and applications in in situ enzyme imaging. This review calls for the development of more activatable QM-based probe with the advantages of high stability in the absence of the target but very high labeling efficiency after activation.

中文翻译：

用于酶原位成像的基于醌甲基化物的自固定分子荧光探针

酶不仅在正常生理过程中发挥着重要作用，而且在许多疾病的发展中也发挥着重要作用。生命系统中酶的高分辨率原位成像将有助于临床诊断和治疗。然而，许多分子荧光探针存在扩散远离酶反应位点甚至细胞外的缺点，丢失原位信息并导致成像分辨率差。基于醌甲基化物 (QM) 的自固定探针可以将荧光信号长时间固定在目标附近，而不会使目标酶失活，从而确保以高分辨率提供放大的信号和目标的原位信息。在这篇综述中，我们总结了基于量子力学的自固定探针的最新进展，包括它们的设计策略、工作机制、分类和在原位酶成像中的应用。该综述呼吁开发更可激活的基于 QM 的探针，其优点是在没有目标的情况下具有高稳定性，但激活后标记效率非常高。

更新日期：2024-03-21

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