We seek to develop techniques for high-resolution imaging of the tree shrew retina for visualizing and parameterizing retinal ganglion cell (RGC) axon bundles in vivo. We applied visible-light optical coherence tomography fibergraphy (vis-OCTF) and temporal speckle averaging (TSA) to visualize individual RGC axon bundles in the tree shrew retina. For the first time, we quantified individual RGC bundle width, height, and cross-sectional area and applied vis-OCT angiography (vis-OCTA) to visualize the retinal microvasculature in tree shrews. Throughout the retina, as the distance from the optic nerve head (ONH) increased from 0.5 mm to 2.5 mm, bundle width increased by 30%, height decreased by 67%, and cross-sectional area decreased by 36%. We also showed that axon bundles become vertically elongated as they converge toward the ONH. Ex vivo confocal microscopy of retinal flat-mounts immunostained with Tuj1 confirmed our in vivo vis-OCTF findings.

中文翻译：

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树鼩视网膜神经节细胞轴突束的可见光光学相干断层扫描纤维成像


我们寻求开发树鼩视网膜高分辨率成像技术，用于体内视网膜神经节细胞（RGC）轴突束的可视化和参数化。我们应用可见光光学相干断层扫描纤维成像 (vis-OCTF) 和颞散斑平均 (TSA) 来可视化树鼩视网膜中的单个 RGC 轴突束。我们首次量化了单个 RGC 束的宽度、高度和横截面积，并应用 vis-OCT 血管造影 (vis-OCTA) 来可视化树鼩的视网膜微脉管系统。在整个视网膜中，随着距视神经头（ONH）的距离从0.5毫米增加到2.5毫米，束宽度增加30％，高度减少67％，横截面积减少36％。我们还表明，轴突束在向 ONH 汇聚时会垂直拉长。用 Tuj1 免疫染色的视网膜平片的离体共聚焦显微镜证实了我们的体内 vis-OCTF 研究结果。