<br>脓毒症相关急性肝损伤的代谢组学和蛋白质组学联合分析及其发病机制研究,International Immunopharmacology

当前位置： X-MOL 学术 › Int. Immunopharmacol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

脓毒症相关急性肝损伤的代谢组学和蛋白质组学联合分析及其发病机制研究
International Immunopharmacology ( IF 4.8 ) Pub Date : 2024-02-26 , DOI: 10.1016/j.intimp.2024.111666
Jin Zhang ₁ , Xibo Wang ₂ , Yahui Peng ₂ , Jieling Wei ₂ , Yinghao Luo ₂ , Feiyu Luan ₂ , Hongxu Li ₃ , Yang Zhou ₂ , Changsong Wang ₂ , Kaijiang Yu ₂

Affiliation

脓毒症引起的急性肝损伤在重症监护病房的患者中很常见。然而，这种情况的确切机制仍不清楚。本研究旨在探讨脓毒症后小鼠肝组织中蛋白质和代谢物的作用和机制，阐明脓毒症相关肝损伤的分子生物学机制。首先，建立脂多糖（LPS）诱导的脓毒症小鼠模型。然后根据小鼠血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）检测以及肝脏组织病理学检查（HE）染色，将脓毒症小鼠分为两组：脓毒症后急性肝损伤组和脓毒症后非急性肝损伤组。对两组小鼠的肝组织进行代谢组学和蛋白质组学分析，以确定显着不同的代谢物和蛋白质。进一步分析代谢组学和蛋白质组学结果，从蛋白质和代谢水平上鉴定与脓毒症相关急性肝损伤发生发展相关的生物学指标和发病机制。共鉴定出14种差异表达蛋白和46种差异表达代谢物。根据蛋白质组学和代谢组学的相关性分析，重组红细胞膜蛋白带4.2（Epb42）和二磷酸腺苷（ADP）可能是脓毒症相关急性肝损伤的关键蛋白和代谢物。通过蛋白质印迹（WB）检测进一步验证差异蛋白Epb42的表达。我们的研究表明差异蛋白Epb42可能是引起脓毒症相关急性肝损伤的关键蛋白，为脓毒症相关急性肝损伤的可能机制提供了新的有价值的信息。

"点击查看英文标题和摘要"

Combined metabolomic and proteomic analysis of sepsis related acute liver injury and its pathogenesis research

Sepsis-induced acute liver injury is common in patients in intensive care units. However, the exact mechanism of this condition remains unclear. The purpose of this study was to investigate the roles and mechanisms of proteins and metabolites in the liver tissue of mice after sepsis and elucidate the molecular biological mechanisms of sepsis-related liver injury. First, a lipopolysaccharide (LPS)-induced sepsis mouse model was established. Then, according to alanine aminotransferase (ALT) and aspartate aminotransferase (AST) detection in mouse serum and liver histopathological examination (HE) staining, the septic mice were divided into two groups: acute liver injury after sepsis and nonacute liver injury after sepsis. Metabolomics and proteomic analyses were performed on the liver tissues of the two groups of mice to identify significantly different metabolites and proteins. The metabolomics and proteomics results were further analysed to identify the biological indicators and pathogenesis related to the occurrence and development of sepsis-related acute liver injury at the protein and metabolite levels. A total of 14 differentially expressed proteins and 46 differentially expressed metabolites were identified. Recombinant Erythrocyte Membrane Protein Band 4.2 (Epb42) and adenosine diphosphate (ADP) may be the key proteins and metabolites responsible for sepsis-related acute liver injury, according to the correlation analysis of proteomics and metabolomics. The expression of the differential protein Epb42 was further verified by western blot (WB) detection. Our study suggests that the differential protein Epb42 may be key proteins causing sepsis-associated acute liver injury, providing new and valuable information on the possible mechanism of sepsis-associated acute liver injury.

更新日期：2024-02-26

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南