Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation, epigenetic factor, and post-translational modification. Previously, we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation. However, whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown. In this study, we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence (DAP-Seq) approach. Electrophoresis mobility shift assay (EMSA) and dual-luciferase reporter assay (DLR) demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes , and . Further analyses of yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and Luciferase complementation imaging (LCI) assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1, and this interaction weakened the MaERF11-mediated transcriptional repression capacity. Collectively, our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.

中文翻译：

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MaERF11 与 E3 泛素连接酶 MaRFA1 的相互作用参与香蕉采后成熟过程中淀粉降解的调节

香蕉果实成熟是一个高度调控的过程，涉及转录调控、表观遗传因子和翻译后修饰等各个层面。此前，我们报道了MaERF11与MaHDA1合作，通过组蛋白去乙酰化精确调控成熟相关基因的转录。然而，MaERF11 在香蕉成熟过程中是否受到翻译后修饰尚不清楚。在这项研究中，我们发现 MaERF11 使用 DNA 亲和纯化序列 (DAP-Seq) 方法靶向淀粉降解相关基因的子集。电泳迁移率变动分析 (EMSA) 和双荧光素酶报告基因分析 (DLR) 表明 MaERF11 可以特异性结合并抑制淀粉降解相关基因的表达。酵母双杂交（Y2H）、双分子荧光互补（BiFC）和荧光素酶互补成像（LCI）检测的进一步分析表明，MaERF11与泛素E3连接酶MaRFA1相互作用，并且这种相互作用削弱了MaERF11介导的转录抑制能力。总的来说，我们的结果表明了一个额外的调控层，其中 MaERF11 调控香蕉果实成熟，并在翻译后修饰水平上扩展果实成熟的调控网络。