Bioelectrochemical systems with present a viable solution for harnessing H/CO mixtures as substrates, employing mediated electron transfer to an infinite electron acceptor in the form of an anode instead of O. Fourteen redox mediators were spectroelectrochemically characterized, and their efficiency was evaluated through screening with in common cuvettes with screen printed electrodes (e-Cuvettes). Key performance indicators, including total turnover number, reduction rate, and growth, were analyzed. Ferricyanide emerged as highly effective for anodic respiration, reaching a total turnover number of 8.38 over 120 h of cultivation. On the other hand, phenazine methosulfate exhibited the highest reduction rate at 2.49 mM h with a total of 5.16 turnovers. Contrary, growth impairment is reported for menadione, possibly leading to deficient anodic electron transfer. The utilization of a broad spectrum of these shuttle molecules highlights the potential for optimizing bioelectrochemical applications involving .

中文翻译：

---

氧化还原介体与 Cupriavidus necator 的相互作用 – 光谱电化学在线分析

生物电化学系统提供了一种利用 H/CO 混合物作为底物的可行解决方案，采用介导的电子转移到阳极而不是 O 形式的无限电子受体。对 14 种氧化还原介体进行了光谱电化学表征，并通过筛选评估了它们的效率在带有丝网印刷电极的普通比色皿中（电子比色皿）。分析了总营业额、减少率和增长等关键绩效指标。铁氰化物对阳极呼吸非常有效，在 120 小时的培养中总周转数达到 8.38。另一方面，吩嗪硫酸甲酯表现出最高的还原率，为 2.49 mM h，总共 5.16 次转换。相反，据报道甲萘醌会损害生长，可能导致阳极电子转移不足。这些穿梭分子的广谱利用凸显了优化涉及的生物电化学应用的潜力。