Intraoperative Resection Guidance and Rapid Pathological Diagnosis of Osteosarcoma using B7H3 Targeted Probe under NIR-II Fluorescence Imaging,Advanced Science

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Intraoperative Resection Guidance and Rapid Pathological Diagnosis of Osteosarcoma using B7H3 Targeted Probe under NIR-II Fluorescence Imaging
Advanced Science ( IF 14.3 ) Pub Date : 2024-03-19 , DOI: 10.1002/advs.202310167
Fanwei Zeng ₁ , Changjian Li ₂ , Han Wang ₁ , Yueqi Wang ₃ , Tingting Ren ₁ , Fangzhou He ₁ , Jie Jiang ₁ , Jiuhui Xu ₁ , Boyang Wang ₁ , Yifan Wu ₁ , Yiyang Yu ₁ , Zhenhua Hu ₃ , Jie Tian _{2,

3} , Shidong Wang ₁ , Xiaodong Tang ₁

Affiliation

Complete removal of all tumor tissue with a wide surgical margin is essential for the treatment of osteosarcoma (OS). However, it's difficult, sometimes impossible, to achieve due to the invisible small satellite lesions and blurry tumor boundaries. Besides, intraoperative frozen-section analysis of resection margins of OS is often restricted by the hard tissues around OS, which makes it impossible to know whether a negative margin is achieved. Any unresected small tumor residuals will lead to local recurrence and worse prognosis. Herein, based on the high expression of B7H3 in OS, a targeted probe B7H3-IRDye800CW is synthesized by conjugating anti-B7H3 antibody and IRDye800CW. B7H3-IRDye800CW can accurately label OS areas after intravenous administration, thereby helping surgeons identify and resect residual OS lesions (<2 mm) and lung metastatic lesions. The tumor-background ratio reaches 4.42 ± 1.77 at day 3. After incubating fresh human OS specimen with B7H3-IRDye800CW, it can specifically label the OS area and even the microinvasion area (confirmed by hematoxylin-eosin [HE] staining). The probe labeled area is consistent with the tumor area shown by magnetic resonance imaging and complete HE staining of the specimen. In summary, B7H3-IRDye800CW has translational potential in intraoperative resection guidance and rapid pathological diagnosis of OS.

中文翻译：

NIR-II荧光成像下B7H3靶向探针术中指导骨肉瘤切除及快速病理诊断

彻底切除所有肿瘤组织并获得较宽的手术切缘对于骨肉瘤（OS）的治疗至关重要。然而，由于看不见的小卫星病灶和模糊的肿瘤边界，实现这一目标很困难，有时是不可能的。此外，术中冰冻切片分析OS切缘往往受到OS周围硬组织的限制，无法得知是否达到阴性切缘。任何未切除的小肿瘤残留都会导致局部复发和预后较差。在此，基于B7H3在OS中的高表达，通过缀合抗B7H3抗体和IRDye800CW合成了靶向探针B7H3-IRDye800CW。 B7H3-IRDye800CW可以在静脉给药后准确标记OS区域，从而帮助外科医生识别并切除残留的OS病灶（<2 mm）和肺转移病灶。第3天肿瘤背景比达到4.42±1.77。将新鲜的人OS标本与B7H3-IRDye800CW一起孵育后，可以特异性标记OS区域甚至微侵袭区域（通过苏木精-伊红[HE]染色证实）。探针标记区域与磁共振成像和标本完整HE染色显示的肿瘤区域一致。综上所述，B7H3-IRDye800CW 在术中切除指导和 OS 快速病理诊断方面具有转化潜力。

更新日期：2024-03-19

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