<br>没食子酸改性聚乙烯亚胺-聚丙烯碳酸酯-聚乙烯亚胺纳米粒子：合成、表征和抗牙周炎评估,ACS Omega

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没食子酸改性聚乙烯亚胺-聚丙烯碳酸酯-聚乙烯亚胺纳米粒子：合成、表征和抗牙周炎评估
ACS Omega ( IF 3.7 ) Pub Date : 2024-03-15 , DOI: 10.1021/acsomega.4c00261
Zunxuan Xie ₁ , Boyang Gao ₁ , Jinyao Liu ₁ , Jiaming He ₁ , Yuyan Liu ₁ , Fengxiang Gao ₂

Affiliation

该研究的目的是开发新型没食子酸（GA）修饰的聚乙烯亚胺（PEI）-聚碳酸亚丙酯（PPC）-PEI（PEPE）两亲性纳米颗粒，并全面评估其作为靶向Toll样受体的抗牙周炎纳米颗粒的特性。 TLR）。第一步是使用分子对接技术评估 GA 与牙周炎核心触发受体 TLR2 和 TLR4/MD2 的结合潜力。随后，我们对合成的 PEPE 纳米粒子进行了核磁共振、透射电子显微镜和动态光散射分析。最后一步，我们研究了 GA-PEPE 纳米粒子的合成结果和体外抗牙周炎特性。研究表明，GA 具有靶向结合 TLR2 和 TLR4/MD2 复合物的潜力。此外，我们成功开发了91.19 nm带正电的PEPE纳米颗粒。光谱分析表明GA修饰的PEPE成功合成。此外，CCK8 结果表明，GA 修饰显着降低了 PEPE 的生物毒性。体外抗牙周炎特性评估表明，6.25 μM GA-PEPE 纳米颗粒显着降低促炎因子 TNF-α、IL-1β 和 IL-6 的表达。 GA-PEPE 纳米颗粒具有靶向 TLR 结合能力，具有优异的生物相容性和抗牙周炎特性。 GA-PEPE纳米颗粒将为抗牙周炎纳米颗粒的开发提供高度创新的投入。

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Gallic Acid-Modified Polyethylenimine–Polypropylene Carbonate–Polyethylenimine Nanoparticles: Synthesis, Characterization, and Anti-Periodontitis Evaluation

The aim of the research was to develop novel gallic acid (GA)-modified amphiphilic nanoparticles of polyethylenimine (PEI)–polypropylene carbonate (PPC)–PEI (PEPE) and comprehensively assess its properties as an antiperiodontitis nanoparticle targeting the Toll-like receptor (TLR). The first step is to evaluate the binding potential of GA to the core trigger receptors TLR2 and TLR4/MD2 for periodontitis using molecular docking techniques. Following this, we conducted NMR, transmission electron microscopy, and dynamic light scattering analyses on the synthesized PEPE nanoparticles. As the final step, we investigated the synthetic results and in vitro antiperiodontitis properties of GA-PEPE nanoparticles. The investigation revealed that GA exhibits potential for targeted binding to TLR2 and the TLR4/MD2 complex. Furthermore, we successfully developed 91.19 nm positively charged PEPE nanoparticles. Spectroscopic analysis indicated the successful synthesis of GA-modified PEPE. Additionally, CCK8 results demonstrated that GA modification significantly reduced the biotoxicity of PEPE. The in vitro antiperiodontitis properties assessment illustrated that 6.25 μM of GA-PEPE nanoparticles significantly reduced the expression of pro-inflammatory factors TNF-α, IL-1β, and IL-6. The GA-PEPE nanoparticles, with their targeted TLR binding capabilities, were found to possess excellent biocompatibility and antiperiodontitis properties. GA-PEPE nanoparticles will provide highly innovative input into the development of anti- periodontitis nanoparticles.

更新日期：2024-03-15

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