Parageobacillus thermoglucosidasius is a thermophilic and facultatively anaerobic microbe, which is emerging as one of the most promising thermophilic model organisms for metabolic engineering. The use of thermophilic microorganisms for industrial bioprocesses provides the advantages of increased reaction rates and reduced cooling costs for bioreactors compared to their mesophilic counterparts. Moreover, it enables starch or lignocellulose degradation and fermentation to occur at the same temperature in a Simultaneous Saccharification and Fermentation (SSF) or Consolidated Bioprocessing (CBP) approach. Its natural hemicellulolytic capabilities and its ability to convert CO to metabolic energy make P. thermoglucosidasius a potentially attractive host for bio-based processes. It can effectively degrade hemicellulose due to a number of hydrolytic enzymes, carbohydrate transporters, and regulatory elements coded from a genomic cluster named Hemicellulose Utilization (HUS) locus. The growing availability of effective genetic engineering tools in P. thermoglucosidasius further starts to open up its potential as a versatile thermophilic cell factory. A number of strain engineering examples showcasing the potential of P. thermoglucosidasius as a microbial chassis for the production of bulk and fine chemicals are presented along with current research bottlenecks. Ultimately, this review provides a holistic overview of the distinct metabolic characteristics of P. thermoglucosidasius and discusses research focused on expanding the native metabolic boundaries for the development of industrially relevant strains.

中文翻译：

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Parageobacillus thermoglucosidasius 作为新兴的嗜热电池工厂


Parageobacillus thermoglucosidasius 是一种嗜热兼性厌氧微生物，正在成为代谢工程中最有前途的嗜热模式生物之一。与嗜温微生物相比，在工业生物工艺中使用嗜热微生物可以提高生物反应器的反应速率并降低冷却成本。此外，它使淀粉或木质纤维素的降解和发酵能够在相同的温度下进行，采用同步糖化和发酵 （SSF） 或综合生物加工 （CBP） 方法。其天然的半纤维素分解能力和将 CO 转化为代谢能的能力使 P. thermoglucosidasius 成为生物基过程的潜在有吸引力的宿主。由于许多水解酶、碳水化合物转运蛋白和调节元件编码自称为半纤维素利用 （HUS） 基因座的基因组簇，它可以有效地降解半纤维素。P. thermoglucosidasius 中有效基因工程工具的日益普及进一步开始开辟其作为多功能嗜热细胞工厂的潜力。介绍了许多菌株工程实例，展示了 P. thermoglucosidasius 作为生产散装和精细化学品的微生物底盘的潜力以及当前的研究瓶颈。最后，这篇综述全面概述了 P. thermoglucosidasius 的独特代谢特征，并讨论了专注于扩大天然代谢边界以开发工业相关菌株的研究。