The biocorrosion process has been a problem for the oil and gas industry, involving deterioration of facilities, loss of oil quality, metal loss, and oil leaks. In oil storage tanks, the presence of a metallic surface along with crude oil and water poses a biocorrosion risk. Biocides are used to control the biocorrosion of different metallic infrastructures, tetrakis(hydroxymethyl)phosphonium sulfate (THPS) is commonly used due to its low environmental impact and its effectiveness in preventing sulfate-reducing bacteria (SRB) growth. The treatment effect with one THPS application on microbial community and corrosion rates was evaluated in a batch fractionated assay containing water from a crude oil storage tank, carbon steel coupons and crude oil. The effects of the treatment were monitored for 60 days. The number of planktonic bacteria and archaea estimated by qPCR decreased at the beginning of the treatment with THPS, however, 60 days after the application their number was higher, as were the sulfate-reducing microorganisms (SRM) and methanogens (MA). The diversity analysis of the 16S rRNA gene showed that the relative abundance of the genus and was higher on day 8 in the presence of THPS, but by day 60 there were no differences. The biocide did not inhibit bacterial and archaeal biofilm attached to the SAE 1010 carbon steel coupons and produced a decrease in corrosion rates by blocking the surface. The proximity of the corrosion potential to the pitting potential indicated a high susceptibility to localized corrosion. The results suggested that the changes in the microbial community by THPS treatment were related to the corrosion rates of carbon steel, rather than the number of microorganisms attached to the coupon.

中文翻译：

---

硫酸四(羟甲基)磷(THPS)对模拟原油储罐环境中微生物群落和碳钢腐蚀的影响

生物腐蚀过程一直是石油和天然气行业面临的一个问题，涉及设施恶化、油质损失、金属损失和石油泄漏。在储油罐中，金属表面以及原油和水的存在会带来生物腐蚀风险。杀菌剂用于控制不同金属基础设施的生物腐蚀，硫酸四（羟甲基）磷（THPS）因其对环境影响较小且能有效防止硫酸盐还原菌（SRB）生长而被广泛使用。在含有来自原油储罐的水、碳钢试样和原油的分批分馏测定中评估了一次 THPS 应用对微生物群落和腐蚀速率的处理效果。治疗效果被监测 60 天。在 THPS 处理开始时，通过 qPCR 估算的浮游细菌和古细菌数量有所减少，但在施用后 60 天，其数量有所增加，硫酸盐还原微生物 (SRM) 和产甲烷菌 (MA) 也是如此。 16S rRNA 基因的多样性分析表明，在 THPS 存在的情况下，该属的相对丰度在第 8 天时较高，但到第 60 天时没有差异。该杀菌剂不会抑制附着在 SAE 1010 碳钢试样上的细菌和古细菌生物膜，并通过堵塞表面来降低腐蚀速率。腐蚀电位接近点蚀电位表明对局部腐蚀的敏感性很高。结果表明，THPS处理后微生物群落的变化与碳钢的腐蚀速率有关，而不是与挂片上附着的微生物数量有关。