Plastic additives such as the antioxidant 2,4-di-tert-butylphenol (2,4-DTBP) have been widely detected in aquatic environments, over a wide range of concentrations reaching 300 μg/L in surface water, potentially threatening the health of aquatic organisms and ecosystems. However, knowledge of the specific effects of 2,4-DTBP on aquatic vertebrates is still limited. In this study, adult zebrafish were exposed to different concentrations of 2,4-DTBP (0, 0.01, 0.1 and 1.0 mg/L) for 21 days in the laboratory. The amplicon sequencing results indicated that the diversity and composition of the zebrafish gut microbiota were significantly changed by 2,4-DTBP, with a shift in the dominant flora to more pathogenic genera. Exposure to 2,4-DTBP at 0.1 and 1.0 mg/L significantly increased the body weight and length of zebrafish, suggesting a biological stress response. Structural assembly defects were also observed in the intestinal tissues of zebrafish exposed to 2,4-DTBP, including autolysis of intestinal villi, adhesions and epithelial detachment of intestinal villi, as well as inflammation. The transcriptional expression of some genes showed that 2,4-DTBP adversely affected protein digestion and absorption, glucose metabolism and lipid metabolism. These results are consistent with the PICRUSt2 functional prediction analysis of intestinal microbiota of zebrafish exposed to 2,4-DTBP. This study improves our understanding of the effects of 2,4-DTBP on the health of aquatic vertebrates and ecosystems.

中文翻译：

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塑料添加剂2,4-二叔丁基苯酚对斑马鱼肠道菌群的影响


抗氧化剂 2,4-二叔丁基苯酚 (2,4-DTBP) 等塑料添加剂已在水生环境中广泛检测到，其浓度范围很广，在地表水中达到 300 μg/L，可能威胁人类的健康。水生生物和生态系统。然而，关于 2,4-DTBP 对水生脊椎动物的具体影响的了解仍然有限。在这项研究中，成年斑马鱼在实验室中暴露于不同浓度的 2,4-DTBP（0、0.01、0.1 和 1.0 mg/L）21 天。扩增子测序结果表明，2,4-DTBP显着改变了斑马鱼肠道微生物群的多样性和组成，优势菌群转向致病性更强的属。暴露于 0.1 和 1.0 mg/L 的 2,4-DTBP 显着增加了斑马鱼的体重和长度，表明存在生物应激反应。暴露于2,4-DTBP的斑马鱼肠道组织也观察到结构组装缺陷，包括肠绒毛自溶、肠绒毛粘连和上皮脱落以及炎症。一些基因的转录表达表明，2,4-DTBP对蛋白质消化吸收、糖代谢和脂质代谢产生不利影响。这些结果与 PICRUSt2 对暴露于 2,4-DTBP 的斑马鱼肠道微生物群的功能预测分析一致。这项研究加深了我们对 2,4-DTBP 对水生脊椎动物和生态系统健康影响的了解。