当前位置：
X-MOL 学术
›
J. Hazard. Mater.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Effects of the plastic additive 2,4-di-tert-butylphenol on intestinal microbiota of zebrafish
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2024-03-08 , DOI: 10.1016/j.jhazmat.2024.133987 Yongmeng Yang 1 , Chen Yan 1 , Aifeng Li 2 , Jiangbing Qiu 2 , Wenhui Yan 1 , Hui Dang 1
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2024-03-08 , DOI: 10.1016/j.jhazmat.2024.133987 Yongmeng Yang 1 , Chen Yan 1 , Aifeng Li 2 , Jiangbing Qiu 2 , Wenhui Yan 1 , Hui Dang 1
Affiliation
|
Plastic additives such as the antioxidant 2,4-di-tert-butylphenol (2,4-DTBP) have been widely detected in aquatic environments, over a wide range of concentrations reaching 300 μg/L in surface water, potentially threatening the health of aquatic organisms and ecosystems. However, knowledge of the specific effects of 2,4-DTBP on aquatic vertebrates is still limited. In this study, adult zebrafish were exposed to different concentrations of 2,4-DTBP (0, 0.01, 0.1 and 1.0 mg/L) for 21 days in the laboratory. The amplicon sequencing results indicated that the diversity and composition of the zebrafish gut microbiota were significantly changed by 2,4-DTBP, with a shift in the dominant flora to more pathogenic genera. Exposure to 2,4-DTBP at 0.1 and 1.0 mg/L significantly increased the body weight and length of zebrafish, suggesting a biological stress response. Structural assembly defects were also observed in the intestinal tissues of zebrafish exposed to 2,4-DTBP, including autolysis of intestinal villi, adhesions and epithelial detachment of intestinal villi, as well as inflammation. The transcriptional expression of some genes showed that 2,4-DTBP adversely affected protein digestion and absorption, glucose metabolism and lipid metabolism. These results are consistent with the PICRUSt2 functional prediction analysis of intestinal microbiota of zebrafish exposed to 2,4-DTBP. This study improves our understanding of the effects of 2,4-DTBP on the health of aquatic vertebrates and ecosystems.
中文翻译：
塑料添加剂 2,4-二叔丁基苯酚对斑马鱼肠道菌群的影响
抗氧化剂 2,4-二叔丁基苯酚 （2,4-DTBP） 等塑料添加剂已在水生环境中广泛检测到，在地表水中浓度范围很广，达到 300 μg/L，可能威胁水生生物和生态系统的健康。然而，关于 2,4-DTBP 对水生脊椎动物的特定影响的了解仍然有限。在这项研究中，成年斑马鱼在实验室中暴露于不同浓度的 2,4-DTBP （0、0.01、0.1 和 1.0 mg/L） 21 天。扩增子测序结果表明，斑马鱼肠道微生物群的多样性和组成受 2,4-DTBP 显着改变，优势菌群向致病性更强的属转移。暴露于 0.1 和 1.0 mg/L 的 2,4-DTBP 显着增加了斑马鱼的体重和长度，表明生物应激反应。在暴露于 2,4-DTBP 的斑马鱼的肠道组织中也观察到结构组装缺陷，包括肠绒毛自溶、肠绒毛粘连和上皮脱离以及炎症。一些基因的转录表达表明，2,4-DTBP 对蛋白质消化吸收、葡萄糖代谢和脂质代谢产生不利影响。这些结果与暴露于 2,4-DTBP 的斑马鱼肠道微生物群的 PICRUSt2 功能预测分析一致。这项研究提高了我们对 2,4-DTBP 对水生脊椎动物和生态系统健康影响的理解。
更新日期：2024-03-08
中文翻译：
塑料添加剂 2,4-二叔丁基苯酚对斑马鱼肠道菌群的影响
抗氧化剂 2,4-二叔丁基苯酚 （2,4-DTBP） 等塑料添加剂已在水生环境中广泛检测到，在地表水中浓度范围很广，达到 300 μg/L，可能威胁水生生物和生态系统的健康。然而，关于 2,4-DTBP 对水生脊椎动物的特定影响的了解仍然有限。在这项研究中，成年斑马鱼在实验室中暴露于不同浓度的 2,4-DTBP （0、0.01、0.1 和 1.0 mg/L） 21 天。扩增子测序结果表明，斑马鱼肠道微生物群的多样性和组成受 2,4-DTBP 显着改变，优势菌群向致病性更强的属转移。暴露于 0.1 和 1.0 mg/L 的 2,4-DTBP 显着增加了斑马鱼的体重和长度，表明生物应激反应。在暴露于 2,4-DTBP 的斑马鱼的肠道组织中也观察到结构组装缺陷，包括肠绒毛自溶、肠绒毛粘连和上皮脱离以及炎症。一些基因的转录表达表明，2,4-DTBP 对蛋白质消化吸收、葡萄糖代谢和脂质代谢产生不利影响。这些结果与暴露于 2,4-DTBP 的斑马鱼肠道微生物群的 PICRUSt2 功能预测分析一致。这项研究提高了我们对 2,4-DTBP 对水生脊椎动物和生态系统健康影响的理解。
京公网安备 11010802027423号