<br>Trachyspermum roxburghianum (DC.) H. Wolff 精油的化学成分、抗氧化、抗菌和伤口愈合作用：体内和计算机方法,Journal of Ethnopharmacology

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Trachyspermum roxburghianum (DC.) H. Wolff 精油的化学成分、抗氧化、抗菌和伤口愈合作用：体内和计算机方法
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2024-03-13 , DOI: 10.1016/j.jep.2024.118055
Mohan Kalaskar ₁ , Anjali Gavit ₁ , Srinivasan Prabhu ₂ , Manish Gagarani ₃ , Vinod Ugale ₄ , Saurabh Khadse ₄ , Muniappan Ayyanar ₅ , Sanjay Surana ₁ , Anilkumar Tatiya ₁ , Shailendra Gurav ₆

Affiliation

(DC.) H. Wolff，俗称“Ajamoda”，是一种被忽视的印度香料，在阿育吠陀和民间传说疗法中广泛使用，作为慢性伤口和分泌物以及许多其他疾病的抗菌剂。该研究的目的是探索印度水果精油的化学成分，并研究其抗氧化、抗菌、镇痛和伤口愈合活性。通过标准定性程序和气相色谱-质谱（GC-MS）技术确定油的植物化学特征。通过清除 DPPH 和 ABTS 自由基来评估抗氧化能力。使用纸片扩散法研究了油的抗菌潜力，然后确定了对革兰氏阳性和革兰氏阴性细菌和真菌菌株的最低抑制浓度。使用白化小鼠的热和化学诱导疼痛模型评估镇痛潜力。使用白化小鼠的切除伤口模型评估伤口愈合，包括确定伤口收缩率、组织病理学和羟脯氨酸估计。 GC-MS 分析鉴定出 55 种主要萜类化合物，包括百里香酚 (13.8%)、柠檬烯 (11.5%) 等。水果精油 (TREO) 表现出显着的自由基清除活性（DPPH 测定中的 IC 为 94.41 ± 2.00 μg/mL，ABTS 测定中的 IC 为 91.28 ± 1.94 μg/mL）。微生物以低 MIC 被抑制（抑制和为 2 μL/mL；抑制 4 μL/mL，抑制 16 μL/mL）。在细胞毒性研究中，在猴正常肾细胞系（Vero）上未观察到细胞毒性。观察到显着的抗伤害作用（100 mg/kg 时抑制率为 25.47 ± 1.10%，200 mg/kg 时抑制率为 44.31 ± 1.69%）。在小鼠伤口愈合过程中，观察到该油的伤口闭合和上皮形成速度显着，同时羟脯氨酸含量显着增加。结果表明，油可以用作伤口愈合疗法的潜在来源。

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Chemical composition, antioxidant, antimicrobial, and wound healing effects of Trachyspermum roxburghianum (DC.) H. Wolff essential oil: An in vivo and in silico approach

(DC.) H. Wolff, commonly known as ‘Ajamoda,’ is a neglected Indian spice highly used in Ayurveda and folklore remedies as an antimicrobial for chronic wounds and discharges, along with many other disease conditions. The objective of the study was to explore chemical composition and to investigate the antioxidant, antimicrobial, analgesic, and wound healing activities of fruit essential oil from India. The phytochemical characterization of the oil was determined through standard qualitative procedures and the gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant aptitude was assessed by scavenging DPPH and ABTS radicals. The antimicrobial potential of the oil was investigated using the disc diffusion method, followed by the determination of minimum inhibitory concentration against Gram-positive and Gram-negative bacterial and fungal strains. The analgesic potential was evaluated using thermal and chemically induced pain models in albino mice. Wound healing was assessed , including determining wound contraction rates, histopathology, and hydroxyproline estimation, using the excision wound model in albino mice. GC-MS analysis identified 55 compounds with major terpenoids, including thymol (13.8%), limonene (11.5%), and others. Substantial radical-scavenging activity was exhibited by fruit essential oil (TREO) (IC 94.41 ± 2.00 μg/mL in DPPH assay and 91.28 ± 1.94 μg/mL in ABTS assay). Microorganisms were inhibited with low MIC (2 μL/mL for the inhibition of and ; 4 μL/mL against and 16 μL/mL against ). In the cytotoxicity study, no cytotoxicity was observed on the Monkey Normal Kidney Cell line (Vero). Significant antinociceptive effects were observed (25.47 ± 1.10 % of inhibition at 100 mg/kg and 44.31 ± 1.69 % at 200 mg/kg). A remarkable rate of wound closure and epithelization, along with a marked increase in hydroxyproline content, were observed for the oil during wound healing in mice. The results suggested that oil could be utilized as a potential source of wound healing therapeutics.

更新日期：2024-03-13

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