Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery to achieve greater, more robust, and more consistent activation is continually under development. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.

中文翻译：

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CRISPR 激活屏幕：导航技术和应用

成簇规则间隔短回文重复序列 (CRISPR) 激活 (CRISPRa) 已成为分子生物学工具包不可或缺的一部分。CRISPRa 遗传筛选是一种令人兴奋的高通量方法，用于识别基因的上调足以引发特定的表型。旨在实现更大、更稳健和更一致的激活的激活机械正在不断开发中。在这篇综述中，我们对可用的 CRISPRa 架构进行了简洁的技术概述，并对汇集的 CRISPRa 筛选进行了全面的总结。此外，我们讨论了 CRISPRa 在广泛研究领域的当代应用，旨在展示 CRISPRa 筛选令人兴奋的新兴应用的观点。