Non-homogeneous enzyme-catalyzed systems are more widely used than homogeneous systems. Distinguished from the conventional biphasic approach, Pickering emulsion stabilized by ultrafine solid particles opens up an innovative platform for biocatalysis. Their vast specific surface area significantly enhances enzyme-substrate interactions, dramatically increasing catalytic efficiency. This review comprehensively explores various aspects of Pickering emulsion biocatalysis, provides insights into the multiple types and mechanisms of its catalysis, and offers strategies for material design, enzyme immobilization, emulsion formation control, and reactor design. Characterization methods are summarized for the determination of drop size, emulsion type, interface morphology, and emulsion potential. Furthermore, recent reports on the design of stimuli-responsive reaction systems are reviewed, enabling the simple control of demulsification. Moreover, the review explores applications of Pickering emulsion in single-step, cascade, and continuous flow reactions and outlines the challenges and future directions for the field. Overall, we provide a review focusing on Pickering emulsions catalysis, which can draw the attention of researchers in the field of catalytic system design, further empowering next-generation bioprocessing.

中文翻译：

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Pickering乳液生物催化：桥接界面设计与酶反应


非均相酶催化系统比均相系统应用更广泛。与传统的双相方法不同，超细固体颗粒稳定的 Pickering 乳液开辟了生物催化的创新平台。它们巨大的比表面积显着增强了酶与底物的相互作用，从而显着提高了催化效率。这篇综述全面探讨了Pickering乳液生物催化的各个方面，深入探讨了其催化的多种类型和机制，并提供了材料设计、酶固定化、乳液形成控制和反应器设计的策略。总结了测定液滴尺寸、乳液类型、界面形态和乳液电位的表征方法。此外，还回顾了最近关于刺激响应反应系统设计的报告，从而实现了破乳的简单控制。此外，该综述还探讨了 Pickering 乳液在单步、级联和连续流反应中的应用，并概述了该领域的挑战和未来方向。总的来说，我们提供了一篇针对皮克林乳液催化的综述，它可以引起催化系统设计领域研究人员的注意，进一步增强下一代生物加工的能力。