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Transcriptomic and biochemical analysis of metabolic remodeling in Bacillus subtilis MSC4 under Benzo[a]pyrene stress
Chemosphere ( IF 8.1 ) Pub Date : 2024-03-08 , DOI: 10.1016/j.chemosphere.2024.141637 Rui Chen 1 , Na Liu 1 , Yuan Ren 2 , Tangbing Cui 1
Chemosphere ( IF 8.1 ) Pub Date : 2024-03-08 , DOI: 10.1016/j.chemosphere.2024.141637 Rui Chen 1 , Na Liu 1 , Yuan Ren 2 , Tangbing Cui 1
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Polyaromatic benzo[]pyrene (B[]P) is a toxic carcinogenic environmental pollutant, and the use of microorganisms to remediate B[]P contamination is considered to be one of the most effective strategies. However, there is still a gap in studying the metabolic remodeling of microorganisms under B[]P stress. In this study, our systematically investigated the effects of B[]P on the metabolism of MSC4 based on transcriptomic, molecular and biochemical analyses. The results showed that in response to B[]P stress, MSC4 formed more biofilm matrix and endospores, the structure of the endospores also was changed, which led to a reduction in their resistance and made them more difficult to germinate. In addition to an increase in glycolysis activity, the activities of tricarboxylic acid cycle, pentose phosphate pathway and the electron transport chain were decreased. B[]P stress forced MSC4 to strengthen arginine synthesis, urea cycle, and urea decomposition, meanwhile, synthesize more ribonucleotides. The activity of DNA replication, transcription activities and the expression of multiple ribosomal protein genes were reduced. Moreover, all of the reported enzymes involved in B[]P degradation showed decreased transcript abundance, and the degradation of B[]P caused significant up-regulation of the gene expression of the acid inducible enzyme OxdC and the synthesis of acetoin. In addition, the cytotoxicity of B[]P to bacteria was directly displayed in four aspects: increased intracellular level of reactive oxygen species (ROS), elevated cell membrane permeability, up-regulation of the cell envelope stress-sensing two-component system LiaRS, and downregulation of siderophores biosynthesis. Finally, B[]P also caused morphological changes in the cells, with some cells exhibiting significant deformation and concavity. These findings provide effective research directions for targeted improvement the cellular activity of B[]P-degrading strains, and is beneficial for further application of microorganisms to remediate B[]P -contaminated soils.
中文翻译：
苯并[a]芘胁迫下枯草芽孢杆菌MSC4代谢重塑的转录组和生化分析
多环苯并[]芘（B[]P）是一种有毒致癌环境污染物，利用微生物修复B[]P污染被认为是最有效的策略之一。然而，B[]P胁迫下微生物代谢重塑的研究仍存在空白。在这项研究中，我们基于转录组学、分子和生化分析系统地研究了 B[]P 对 MSC4 代谢的影响。结果表明，在B[]P胁迫下，MSC4形成更多的生物膜基质和内生孢子，内生孢子的结构也发生改变，导致其抵抗力降低，更难发芽。除了糖酵解活性增加外，三羧酸循环、磷酸戊糖途径和电子传递链的活性也降低。 B[]P应激迫使MSC4加强精氨酸合成、尿素循环和尿素分解，同时合成更多的核糖核苷酸。 DNA复制活性、转录活性和多种核糖体蛋白基因的表达均降低。此外，所有报道的参与 B[]P 降解的酶均表现出转录本丰度下降，并且 B[]P 的降解导致酸诱导酶 OxdC 的基因表达和乙偶姻的合成显着上调。此外，B[]P对细菌的细胞毒性直接表现在四个方面：增加细胞内活性氧（ROS）水平、升高细胞膜通透性、上调细胞包膜应激传感双组分系统LiaRS和铁载体生物合成的下调。 最后，B[]P还引起细胞形态变化，一些细胞表现出明显的变形和凹陷。这些研究结果为有针对性地提高苯并[]芘降解菌株的细胞活性提供了有效的研究方向，有利于进一步应用微生物修复苯并[]芘污染土壤。
更新日期：2024-03-08
中文翻译：
苯并[a]芘胁迫下枯草芽孢杆菌MSC4代谢重塑的转录组和生化分析
多环苯并[]芘（B[]P）是一种有毒致癌环境污染物，利用微生物修复B[]P污染被认为是最有效的策略之一。然而，B[]P胁迫下微生物代谢重塑的研究仍存在空白。在这项研究中，我们基于转录组学、分子和生化分析系统地研究了 B[]P 对 MSC4 代谢的影响。结果表明，在B[]P胁迫下，MSC4形成更多的生物膜基质和内生孢子，内生孢子的结构也发生改变，导致其抵抗力降低，更难发芽。除了糖酵解活性增加外，三羧酸循环、磷酸戊糖途径和电子传递链的活性也降低。 B[]P应激迫使MSC4加强精氨酸合成、尿素循环和尿素分解，同时合成更多的核糖核苷酸。 DNA复制活性、转录活性和多种核糖体蛋白基因的表达均降低。此外，所有报道的参与 B[]P 降解的酶均表现出转录本丰度下降，并且 B[]P 的降解导致酸诱导酶 OxdC 的基因表达和乙偶姻的合成显着上调。此外，B[]P对细菌的细胞毒性直接表现在四个方面：增加细胞内活性氧（ROS）水平、升高细胞膜通透性、上调细胞包膜应激传感双组分系统LiaRS和铁载体生物合成的下调。 最后，B[]P还引起细胞形态变化，一些细胞表现出明显的变形和凹陷。这些研究结果为有针对性地提高苯并[]芘降解菌株的细胞活性提供了有效的研究方向，有利于进一步应用微生物修复苯并[]芘污染土壤。
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