<br>研究牛磺熊去氧胆酸 (TUDCA) 在减轻小白菜内质网应激和细胞反应中的作用,Physiologia Plantarum

当前位置： X-MOL 学术 › Physiol. Plant › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

研究牛磺熊去氧胆酸 (TUDCA) 在减轻小白菜内质网应激和细胞反应中的作用
Physiologia Plantarum ( IF 5.4 ) Pub Date : 2024-03-12 , DOI: 10.1111/ppl.14246
Aliya Ayaz ₁ , Abdul Jalal ₂ , Zhou Qian ₁ , Khalid Ali Khan ₃ , Liwang Liu ₁ , Chunmei Hu ₁ , Ying Li ₁ , Xilin Hou ₁

Affiliation

由于不利条件，植物细胞内质网 (ER) 中错误折叠的蛋白质积累会导致 ER 应激。这会激活涉及活性氧 (ROS) 和未折叠蛋白反应 (UPR) 的相互关联的通路，这些通路在调节 ER 应激中发挥着至关重要的作用。本研究的目的是研究衣霉素 (TM) 诱导 ER 应激的潜在机制，并探索牛磺熊去氧胆酸 (TUDCA) 在减轻白菜细胞对 ER 应激的反应方面的潜在治疗应用。油菜亚种。中华草）。研究表明，小白菜的内质网应激会对植物形态、活性氧水平、细胞膜完整性和抗氧化防御系统产生不利影响。然而，在 TM 诱导的 ER 应激小白菜中使用 TUDCA 处理可改善形态指标、色素含量、ROS 积累、细胞膜完整性和抗氧化防御系统恢复。此外，TUDCA 还在小白菜 ER 应激期间调节 ER 应激传感器基因、ER 伴侣基因和 ER 相关降解 (ERAD) 基因的转录水平。此外，TUDCA 已证明其能够缓解 ER 应激、稳定 UPR、减少氧化应激、防止细胞凋亡并对植物生长和发育产生积极影响。这些结果共同表明 TUDCA 是一种有前途的减轻小白菜植物 ER 胁迫引起的损伤的药物，并为作物保护和胁迫管理的进一步研究和潜在应用提供了宝贵的见解。

"点击查看英文标题和摘要"

Investigating the effects of tauroursodeoxycholic acid (TUDCA) in mitigating endoplasmic reticulum stress and cellular responses in Pak choi

The accumulation of misfolded proteins in the endoplasmic reticulum (ER) within plant cells due to unfavourable conditions leads to ER stress. This activates interconnected pathways involving reactive oxygen species (ROS) and unfolded protein response (UPR), which play vital roles in regulating ER stress. The aim of this study is to investigate the underlying mechanisms of tunicamycin (TM) induced ER stress and explore the potential therapeutic applications of tauroursodeoxycholic acid (TUDCA) in mitigating cellular responses to ER stress in Pak choi (Brassica campestris subsp. chinensis). The study revealed that ER stress in Pak choi leads to detrimental effects on plant morphology, ROS levels, cellular membrane integrity, and the antioxidant defence system. However, treatment with TUDCA in TM‐induced ER stressed Pak choi improved morphological indices, pigment contents, ROS accumulation, cellular membrane integrity, and antioxidant defence system restoration. Additionally, TUDCA also modulates the transcription levels of ER stress sensors genes, ER chaperone genes, and ER‐associated degradation (ERAD) genes during ER stress in Pak choi. Furthermore, TUDCA has demonstrated its ability to alleviate ER stress, stabilize the UPR, reduce oxidative stress, prevent apoptosis, and positively influence plant growth and development. These results collectively comprehend TUDCA as a promising agent for mitigating ER stress‐induced damage in Pak choi plants and provide valuable insights for further research and potential applications in crop protection and stress management.

更新日期：2024-03-12

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南