<br>基于 Gd-MOF 的无共反应物电化学发光发光体和 [Ru(bpy)2dppz]2+ 的“光开关”分子的用于灵敏啶虫脒分析的电位分辨比率适体传感器,Analytical Chemistry

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基于 Gd-MOF 的无共反应物电化学发光发光体和 [Ru(bpy)2dppz]2+ 的“光开关”分子的用于灵敏啶虫脒分析的电位分辨比率适体传感器
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-03-12 , DOI: 10.1021/acs.analchem.4c00364
Jingshuai Li ₁ , Yan Zhou ₁ , Mengzhen Xi ₁ , Liuyong Hu ₂ , Bingzhang Lu ₃ , Wenling Gu ₁ , Chengzhou Zhu _{1,

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Affiliation

对于传统的电位分辨比率电化学发光 (ECL) 系统，必须引入多重共反应物。然而，不同共反应物之间的不良相互作用不可避免地影响分析精度和灵敏度。在此，通过聚集诱导发射配体与钆阳离子的配位，制备了自发光金属有机框架（Gd-MOF）并作为一种新型的无共反应物阳极ECL发射体。通过将具有光开关效应的[Ru(bpy) ₂ dppz] ²⁺嵌入DNA双链体中，以K ₂ S ₂ O ₈为共反应剂，得到一种高效阴极ECL探针。在啶虫脒存在的情况下，靶标与其适体之间的强亲和力诱导 [Ru(bpy) ₂ dppz] ²⁺的释放，由于 Gd 的 ECL 共振能量转移，导致阴极信号减少和阳极信号增加-MOF 至 [Ru(bpy) ₂ dppz] ²⁺ 。通过这种方式，构建了一种高效的双信号 ECL 适体传感器，用于啶虫脒的比率分析，并表现出 0.033 pM 的极低检测限。引人注目的是，通过仅使用一种外源共反应物，可以消除多种共反应物的交叉干扰，从而提高检测精度。所开发的高性能ECL传感平台成功应用于实际样品中啶虫脒残留水平的监测，展示了其在食品安全领域的潜在应用。

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Potential-Resolved Ratiometric Aptasensor for Sensitive Acetamiprid Analysis Based on Coreactant-free Electrochemiluminescence Luminophores of Gd-MOF and “Light Switch” Molecule of [Ru(bpy)2dppz]2+

For conventional potential-resolved ratiometric electrochemiluminescence (ECL) systems, the introduction of multiplex coreactants is imperative. However, the undesirable interactions between different coreactants inevitably affect analytical accuracy and sensitivity. Herein, through the coordination of aggregation-induced emission ligands with gadolinium cations, the self-luminescent metal–organic framework (Gd-MOF) is prepared and serves as a novel coreactant-free anodic ECL emitter. By the intercalation of [Ru(bpy)₂dppz]²⁺ with light switch effect into DNA duplex, one high-efficiency cathodic ECL probe is obtained using K₂S₂O₈ as a coreactant. In the presence of acetamiprid, the strong affinity between the target and its aptamer induces the release of [Ru(bpy)₂dppz]²⁺, resulting in a decreasing cathode signal and an increasing anode signal owing to the ECL resonance energy transfer from Gd-MOF to [Ru(bpy)₂dppz]²⁺. In this way, an efficient dual-signal ECL aptasensor is constructed for the ratiometric analysis of acetamiprid, exhibiting a remarkably low detection limit of 0.033 pM. Strikingly, by using only one exogenous coreactant, the cross interference from multiple coreactants can be eliminated, thus improving the detection accuracy. The developed high-performance ECL sensing platform is successfully applied to monitor the residual level of acetamiprid in real samples, demonstrating its potential application in the field of food security.

更新日期：2024-03-12

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