In quantifying NH and/or NH ions, the wavelength-based spectrochemical methods involving formation of colored products of the Berthelot’s or Nessler reactions might be challenging due to auto-self absorbance, spectral overlap, and background scattering noise Thus, the current study reports a renewable glassy carbon electrode (GCE) sensing probe combined with Berthelot’s reaction (indophenol formation) and adsorptive square - wave-anodic stripping voltammetry (Ads SWSV) at pH = 10 for detection of NH and/or NH in water. The redox characteristics and the high surface coverage of the oxidation product of indophenol on the sensing platform suggested its use for NH and/or NH detection. The electrochemical sensing probe for NH displayed good linear relationship between 5.56 nM and 55.6 μM of NH with limits of detection (LOD) and quantitation (LOQ) of 4.83 × 10 and 1.47 × 10 M, and sensing probe sensitivity of 1.27μA/μM cm,respectively. The probe was applied for measuring NH/NHin fresh and seawater samples, and the results were validated using standard ion chromatography (IC) and micro spectrophotometry assays. The assembled probe was also tolerably selective against interfering of other contaminants in a comparable potential window. Additionally, the probe has exceptional selectivity, long-term stability, and repeatability, and has good capacity to detect NH and/or NH ions with high accuracy (recovery range = 97.14 ± 4.12–102.9 ± 4.7) in environmental water samples. The calculated Student and values (n = 5) were less than the tabulated (2.78) and (6.39) at 95 % probability = 0.05, n = 5). The study offer high reliability towards NH and/or NH detection in complex environments.

中文翻译：

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结合了经典 Berthelot 反应和玻碳电极的高灵敏度电化学传感探头，用于测量水中超痕量的氨/ NH4+

在定量 NH 和/或 NH 离子时，由于自吸收、光谱重叠和背景散射噪声，涉及 Berthelot 或 Nessler 反应有色产物形成的基于波长的光谱化学方法可能具有挑战性。可再生玻碳电极 (GCE) 传感探针与 Berthelot 反应（靛酚形成）和吸附方波阳极溶出伏安法 (Ads SWSV) 相结合，在 pH = 10 时检测水中的 NH 和/或 NH。传感平台上靛酚氧化产物的氧化还原特性和高表面覆盖率表明其可用于 NH 和/或 NH 检测。 NH 电化学传感探针在 5.56 nM 和 55.6 μM NH 之间表现出良好的线性关系，检测限 (LOD) 和定量限 (LOQ) 分别为 4.83 × 10 和 1.47 × 10 M，传感探针灵敏度为 1.27μA/μM cm ，分别。该探针用于测量淡水和海水样品中的 NH3/NH3，并使用标准离子色谱 (IC) 和微分光光度测定法对结果进行验证。组装好的探针在相当的电位窗口中对其他污染物的干扰也具有良好的选择性。此外，该探头具有出色的选择性、长期稳定性和重复性，并且能够以高精度（回收范围 = 97.14 ± 4.12–102.9 ± 4.7）检测环境水样中的 NH 和/或 NH 离子。计算出的 Student 和值 (n = 5) 小于表中的 (2.78) 和 (6.39)（95% 概率 = 0.05，n = 5）。该研究为复杂环境中的 NH 和/或 NH 检测提供了高可靠性。