Electrochemical manipulation of enzymes is expected to enable the rapid detection of marker molecules and the efficient production of valuable materials. We have fabricated an Au nanoparticle (AuNP) thin film electrode in our previous study, which enables more enhanced heterogeneous electron transfer reactions than a conventional planner Au electrode. In this study, electrochemical evaluation of cytochrome was performed using the AuNP thin film electrodes with and without self-assembled monolayer (SAM) modification to assess its practicality for protein electrochemistry. The 4-pyridinethiol- and 7-carboxy-1-heptanethiol-modified AuNP thin film electrodes showed much larger faradaic current values attributed to redox reactions compared to those of the Au electrodes. The results indicate that the nanostructural effects of the AuNP thin film electrode are beneficial. Importantly, even when the non-modified (bare) AuNP thin film electrode was applied, it exhibited the clear electrochemical response of cyt , while the bare Au electrode showed no such a response. This study provides evidence that the AuNP thin film electrode functions as a potent bioelectrocatalysts due to the nanostructure-specific properties.

中文翻译：

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无帽金纳米粒子薄膜作为直接电子转移至氧化还原蛋白的电极的功效

酶的电化学操作有望实现标记分子的快速检测和有价值材料的高效生产。我们在之前的研究中制造了一种金纳米颗粒（AuNP）薄膜电极，与传统的平面金电极相比，它能够实现更增强的异质电子转移反应。在本研究中，使用具有和不具有自组装单层 (SAM) 修饰的 AuNP 薄膜电极对细胞色素进行电化学评估，以评估其在蛋白质电化学中的实用性。与 Au 电极相比，4-吡啶硫醇和 7-羧基-1-庚硫醇修饰的 AuNP 薄膜电极表现出更大的归因于氧化还原反应的法拉第电流值。结果表明，AuNP 薄膜电极的纳米结构效应是有益的。重要的是，即使应用未修饰的（裸）AuNP 薄膜电极，它也表现出清晰的细胞色素电化学响应，而裸Au 电极则没有表现出这样的响应。这项研究提供了证据，证明 AuNP 薄膜电极由于纳米结构的特定特性而可以作为有效的生物电催化剂。