<br>揭示 ONOO− 检测：生物系统快速响应荧光探针的出现,Journal of Materials Chemistry B

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揭示 ONOO− 检测：生物系统快速响应荧光探针的出现
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2024-03-12 , DOI: 10.1039/d3tb02994h
Guoliang Wu ₁ , Zihong Li ₁ , Ping Huang ₁ , Weiying Lin ₁

Affiliation

ONOO ^-是一种生物活性分子，在炎症相关信号通路和病理机制中发挥着关键作用。大量研究已证实^ONOO−水平升高与肿瘤进展之间存在直接相关性。因此，研究炎症和肿瘤中的^ONOO−水平至关重要。荧光成像为 ONOO ^-原位检测提供了一种高灵敏度、非侵入性、易于操作、选择性和高效的方法。在本研究中，我们设计并合成了一种基于罗丹明的探针NRho ，它通过检测ONOO ^-含量来有效识别肿瘤、炎症细胞、组织和器官。 NRho的合成过程简单，得到的探针具有良好的光谱特性和快速的响应。我们的细胞成像分析提供了新的见解，揭示了不同类型癌细胞之间不同的 ONOO ^-水平，其中肝细胞癌细胞比其他细胞表现出更高的 ONOO ^-含量。这一观察结果标志着不同癌细胞类型之间^ONOO−水平存在差异的提议。此外，我们的研究展示了我们的探针在活体器官成像中的实用性，能够在短短 5 分钟的潜伏期内识别活体器官中的肿瘤。此外，我们的研究结果强调了探针NRho在各种组织样本中的快速检测能力，可有效识别炎症。这项研究对于推进生物医学研究和临床诊断具有重要前景。

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Shedding light on ONOO− detection: the emergence of a fast-response fluorescent probe for biological systems

ONOO⁻, a bioactive molecule, plays a critical role in inflammation-related signaling pathways and pathological mechanisms. Numerous studies have established a direct correlation between elevated ONOO⁻ levels and tumor progression. Therefore, investigating ONOO⁻ levels in inflammation and tumors is of utmost importance. Fluorescence imaging presents a highly sensitive, non-invasive, easily operable, selective, and efficient method for ONOO⁻ detection in situ. In this study, we designed and synthesized a rhodamine-based probe, NRho, which effectively identifies tumors, inflammatory cells, tissues, and organs by detecting ONOO⁻ content. The synthesis process of NRho is simple, yielding a probe with favorable spectral characteristics and rapid response. Our cell imaging analysis has provided novel insights, revealing distinct ONOO⁻ levels among different types of cancer cells, with hepatocellular carcinoma cells exhibiting higher ONOO⁻ content than the others. This observation marks the proposal of such variations in ONOO⁻ levels across cancer cell types. Furthermore, our study has showcased the practicality of our probe in live organ imaging, enabling the identification of tumors from living organs within a brief 5-minute incubation period. Additionally, our findings highlight the rapid detection capability of the probe NRho in various tissue samples, effectively identifying inflammation. This research holds important promise in advancing biomedical research and clinical diagnosis.

更新日期：2024-03-12

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