<br>壳聚糖寡糖通过促进共轭质粒转移加速抗生素抗性基因的传播,Journal of Hazardous Materials

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壳聚糖寡糖通过促进共轭质粒转移加速抗生素抗性基因的传播
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2024-03-01 , DOI: 10.1016/j.jhazmat.2024.133922
Yan Zhang ₁ , Wenjie Li ₁ , Yashuang Wu ₁ , Xiaomin Tian ₁ , Gong Li ₁ , Yufeng Zhou ₂ , Jian Sun ₂ , Xiaoping Liao ₂ , Yahong Liu ₃ , Yang Wang ₄ , Yang Yu ₂

Affiliation

抗生素耐药基因（ARG）的传播，特别是通过质粒介导的水平基因转移，对全球健康构成了普遍的威胁。壳聚糖-低聚糖（COS）广泛用于医学、植物和畜牧业。然而，它们对微生物群落的影响意味着有可能对质粒转移施加选择性压力。为了探索 COS 在通过质粒偶联促进 ARGs 传播中的作用，我们建立了体外交配模型。将 COS 添加到偶联混合物中显着增强了 RP4 质粒和阳性 Inc 质粒在种内和种间的转移。表型和转录组分析显示，COS 通过中和细胞表面电荷和增加细胞表面疏水性来增强细胞间接触。此外，COS 通过抑制 Tol-Pal 系统来提高膜通透性，从而促进质粒偶联转移。此外，COS 作为碳源并被代谢，通过调节 RP4 质粒中 ATP 酶和全局阻遏因子相关基因的表达为质粒偶联提供能量。总体而言，这些发现提高了我们对与 COS 的存在和细菌抗生素耐药性传播相关的潜在风险的认识，强调了为谨慎使用 COS 及其排放到环境中建立指南的必要性。

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Chitosan oligosaccharide accelerates the dissemination of antibiotic resistance genes through promoting conjugative plasmid transfer

The dissemination of antibiotic resistance genes (ARGs), especially via plasmid-mediated horizontal gene transfer, poses a pervasive threat to global health. Chitosan-oligosaccharide (COS) is extensively utilized in medicine, plant and animal husbandry. However, their impact on microflora implies the potential to exert selective pressure on plasmid transfer. To explore the role of COS in facilitating the dissemination of ARGs via plasmid conjugation, we established in vitro mating models. The addition of COS to conjugation mixtures significantly enhanced the transfer of RP4 plasmid and positive Inc plasmid in both intra- and inter-specific. Phenotypic and transcriptome analysis revealed that COS enhanced intercellular contact by neutralizing cell surface charge and increasing cell surface hydrophobicity. Additionally, COS increased membrane permeability by inhibiting the Tol-Pal system, thereby facilitating plasmid conjugative transfer. Furthermore, COS served as the carbon source and was metabolized by , providing energy for plasmid conjugation through regulating the expression of ATPase and global repressor factor-related genes in RP4 plasmid. Overall, these findings improve our awareness of the potential risks associated with the presence of COS and the spread of bacterial antibiotic resistance, emphasizing the need to establish guidelines for the prudent use of COS and its discharge into the environment.

更新日期：2024-03-01

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