CRISPR-Cas systems revolutionized the genome engineering field but need to induce double-strand breaks (DSBs) and may be difficult to deliver due to their large protein size. Tn7-like transposons such as CRISPR-associated transposons (CASTs) can be repurposed for RNA-guided DSB-free integration, and obligate mobile element guided activity (OMEGA) proteins of the IS200/IS605 transposon family have been developed as hypercompact RNA-guided genome editing tools. CASTs and OMEGA are exciting, innovative genome engineering tools that can improve the precision and efficiency of editing. This review explores the recent developments and uses of CASTs and OMEGA in genome editing across prokaryotic and eukaryotic cells. The pros and cons of these transposon-based systems are deliberated in comparison to other CRISPR systems.

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RNA引导的基因组工程：向转座子的范式转变

CRISPR-Cas 系统彻底改变了基因组工程领域，但需要诱导双链断裂 (DSB)，并且由于其蛋白质尺寸较大，可能难以实现。Tn7 样转座子，如 CRISPR 相关转座子 (CAST) 可重新用于 RNA 引导的无 DSB 整合，IS200/IS605 转座子家族的专性移动元件引导活性 (OMEGA) 蛋白已被开发为超紧凑 RNA 引导的转座子。基因组编辑工具。CAST 和 OMEGA 是令人兴奋的创新基因组工程工具，可以提高编辑的精度和效率。本综述探讨了 CAST 和 OMEGA 在原核和真核细胞基因组编辑中的最新发展和用途。与其他 CRISPR 系统相比，我们讨论了这些基于转座子的系统的优缺点。