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Glycyrrhizic acid treatment ameliorates anxiety-like behaviour via GLT1 and Per1/2-dependent pathways
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2024-03-05 , DOI: 10.1016/j.jep.2024.118013
Shanbo Ma 1 , Ye Chong 2 , Rui Zhang 3 , Wei Quan 4 , Jiayue Gui 5 , Long Li 1 , Jin Wang 1 , Shan Miao 1 , Xiaopeng Shi 1 , Minggao Zhao 6 , Kun Zhang 5
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2024-03-05 , DOI: 10.1016/j.jep.2024.118013
Shanbo Ma 1 , Ye Chong 2 , Rui Zhang 3 , Wei Quan 4 , Jiayue Gui 5 , Long Li 1 , Jin Wang 1 , Shan Miao 1 , Xiaopeng Shi 1 , Minggao Zhao 6 , Kun Zhang 5
Affiliation
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As a traditional Chinese medicinal herb, . Fisch. (licorice root, chinese name: Gancao) has a variety of medicinal values and is widely used clinically. Its main active ingredient, glycyrrhizic acid (GA), is believed to have a neuroprotective effect. However, the underlying biological mechanisms of GA on stress-induced anxiety disorders are still unclear. To investigate the anti-anxiety effect of GA and its underlying mechanism. We selected the anxiety model induced by repeated chronic restraint stress (CRS) for 2 h on each of 7 consecutive days. GA (4, 20, 100 mg/kg) was injected intraperitoneally once daily for 1 week. The potential GA receptors were identified using whole-cell patches and computer-assisted docking of molecules. High-throughput RNA sequencing, adeno-associated virus-mediated gene regulation, Western blotting, and RT-qPCR were used to assess the underlying molecular pathways. GA alleviate depression-like and anxiety-like behaviors in CRS mice. GA decreased synaptic transmission by facilitating glutamate reuptaking in mPFC. Meanwhile, long-term GA treatment increased the expression of clock genes and . Suppressing both and abolished the anxiolytic effects of GA treatment. Our study suggests that GA may be developed for the treatment of stress-induced anxiety disorders, and its mechanism is related to GLT1 and Per1/2-dependent pathways. This presents a novel approach to discovering potent therapeutic drugs.
中文翻译：
甘草酸治疗通过 GLT1 和 Per1/2 依赖性途径改善焦虑样行为
作为一种传统的中药材，。费施。 （甘草，中药名：甘草）具有多种药用价值，临床应用广泛。其主要活性成分甘草酸（GA）被认为具有神经保护作用。然而，GA 治疗应激性焦虑症的潜在生物学机制仍不清楚。探讨GA的抗焦虑作用及其机制。我们选择连续7天每天重复2小时的慢性束缚应激（CRS）诱发的焦虑模型。腹腔注射GA（4、20、100 mg/kg），每天一次，持续1周。使用全细胞贴片和计算机辅助分子对接来鉴定潜在的 GA 受体。高通量 RNA 测序、腺相关病毒介导的基因调控、蛋白质印迹和 RT-qPCR 用于评估潜在的分子途径。 GA 可以减轻 CRS 小鼠的抑郁样和焦虑样行为。 GA 通过促进 mPFC 中谷氨酸的再摄取来减少突触传递。同时，长期GA处理增加了时钟基因和的表达。抑制两者并消除 GA 治疗的抗焦虑作用。我们的研究表明，GA可能被开发用于治疗应激性焦虑症，其机制与GLT1和Per1/2依赖性途径有关。这提供了一种发现有效治疗药物的新方法。
更新日期：2024-03-05
中文翻译：
甘草酸治疗通过 GLT1 和 Per1/2 依赖性途径改善焦虑样行为
作为一种传统的中药材，。费施。 （甘草，中药名：甘草）具有多种药用价值，临床应用广泛。其主要活性成分甘草酸（GA）被认为具有神经保护作用。然而，GA 治疗应激性焦虑症的潜在生物学机制仍不清楚。探讨GA的抗焦虑作用及其机制。我们选择连续7天每天重复2小时的慢性束缚应激（CRS）诱发的焦虑模型。腹腔注射GA（4、20、100 mg/kg），每天一次，持续1周。使用全细胞贴片和计算机辅助分子对接来鉴定潜在的 GA 受体。高通量 RNA 测序、腺相关病毒介导的基因调控、蛋白质印迹和 RT-qPCR 用于评估潜在的分子途径。 GA 可以减轻 CRS 小鼠的抑郁样和焦虑样行为。 GA 通过促进 mPFC 中谷氨酸的再摄取来减少突触传递。同时，长期GA处理增加了时钟基因和的表达。抑制两者并消除 GA 治疗的抗焦虑作用。我们的研究表明，GA可能被开发用于治疗应激性焦虑症，其机制与GLT1和Per1/2依赖性途径有关。这提供了一种发现有效治疗药物的新方法。
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