西花蓟马（缨翅目：蓟马科）丝氨酸/苏氨酸激酶 AKT 和磷酸肌醇依赖性激酶 PDK 对 3 种食物的响应及其对生殖功能的调节,Insect Molecular Biology

当前位置： X-MOL 学术 › Insect Mol. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

西花蓟马（缨翅目：蓟马科）丝氨酸/苏氨酸激酶 AKT 和磷酸肌醇依赖性激酶 PDK 对 3 种食物的响应及其对生殖功能的调节
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2024-03-07 , DOI: 10.1111/imb.12905
Xinyue Qiu ₁ , Wanqing Huang _{1,

2} , Wenbo Yue ₁ , Dingyin Li ₁ , Junrui Zhi ₁

Affiliation

西花蓟马Pergande（Pergande）是一种典型的杂食性昆虫，以寄主植物、花粉和螨卵为食，对全世界农作物构成威胁。胰岛素信号通路（ISP）是典型的营养敏感通路，参与昆虫多种功能的调节。丝氨酸/苏氨酸激酶（AKT）和磷酸肌醇依赖性激酶（PDK）是 ISP 的关键组件。在这项研究中，福阿克特和FOPDK基因在西方镰刀菌克隆了它们，并确定了三种食物对其表达的影响。的表达式为福阿克特和FOPDK以补充有松花粉或螨虫卵的芸豆叶为食的蓟马的含量高于主要以单独叶子为食的蓟马。同时，以补充松花粉的叶子为食的蓟马的繁殖力最高。此外，RNA干扰介导的基因敲低福阿克特和FOPDK卵黄蛋白原 (Vg) 含量降低电压在雌性中表达，卵巢长度缩短，卵子发育延迟，生育力和后代孵化率降低。此外，保幼激素（JH）的合成减少，葡萄糖、海藻糖、糖原和海藻糖酶的含量受到影响。这些结果表明福阿克特和FOPDK调节繁殖西方镰刀菌通过调节 Vg 和 JH 的产生以及碳水化合物代谢。

"点击查看英文标题和摘要"

Response of the serine/threonine kinase AKT and phosphoinositide‐dependent kinase PDK in Frankliniella occidentalis (Thysanoptera: Thripidae) to three kinds of foods and their regulation of reproductive function

Frankliniella occidentalis (Pergande) is a typical omnivorous insect that feeds on host plants, pollens and mite eggs, and poses a threat to crops worldwide. The insulin signalling pathway (ISP) is a typical nutrient‐sensitive pathway that participates in the regulation of various functions in insects. Serine/threonine kinases (AKTs) and phosphoinositide‐dependent kinases (PDKs) are key components of the ISP. In this study, the FoAKT and FoPDK genes in F. occidentalis were cloned, and the effects of three foods on their expression were determined. The expression of FoAKT and FoPDK in the thrips fed on kidney bean leaves supplemented with pine pollen or mite eggs was higher than in those primarily fed on leaves alone. Meanwhile, the fecundity of thrips fed on leaves supplemented with pine pollen was highest. In addition, RNA interference‐mediated knockdown of FoAKT and FoPDK decreased vitellogenin (Vg) content and Vg expression in females, shortened ovariole length, delayed egg development and reduced fecundity and offspring hatching rates. Furthermore, the synthesis of juvenile hormone (JH) was reduced, and the contents of glucose, trehalose, glycogen and trehalase were affected. These results suggest that FoAKT and FoPDK regulate the reproduction of F. occidentalis by regulating Vg and JH production as well as carbohydrate metabolism.

更新日期：2024-03-07

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>