集成光阴极与光阳极的阴极光电化学免疫传感平台,Analytical Chemistry

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集成光阴极与光阳极的阴极光电化学免疫传感平台
Analytical Chemistry ( IF 6.7 ) Pub Date : 2016-10-19 00:00:00 , DOI: 10.1021/acs.analchem.6b03473
Gao-Chao Fan ₁ , Xiao-Mei Shi ₁ , Jian-Rong Zhang _{1,

2} , Jun-Jie Zhu ₁

Affiliation

通常，基于光阳极的光电化学免疫分析法具有明显的光电流响应和较低的理想样品检测下限，但固有的缺陷是其对真实样品的抗干扰能力较差。基于光阴极的免疫测定可以很好地避免基于光阳极的免疫测定的固有缺点，但是它的光电流响应较低，因此灵敏度较差。在此，报道了将光阴极与光阳极结合的有希望的新的阴极光电化学免疫传感平台，用于生物标志物的准确和灵敏的检测。在该建议中，选择了前列腺特异性抗原（PSA，Ag）作为目标分析物的模型，以展示该平台的分析性能。二氧化钛₂/ CdS：Mn杂化结构修饰的铟锡氧化物（ITO）电极用作光阳极，而CuInS ₂选择微花修饰的ITO电极作为光电阴极。在光阴极上修饰PSA抗体（Ab）的换能器元件，以制造无标记的阴极免疫传感电极。所提出的免疫传感平台同时具有两个明显的优点。首先，由于生物识别事件发生在光电阴极上，因此它对于检测真实的生物样品具有良好的抗干扰能力。其次，由于引入了光电阳极，因此光电化学系统具有明显的光电流响应和对目标Ag检测的低检测限。此外，所提出的免疫传感平台还具有良好的特异性，可重复性和稳定性，同时为构建其他类型的光电化学生物传感器开辟了新的视野。

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Cathode Photoelectrochemical Immunosensing Platform Integrating Photocathode with Photoanode

Generally, photoanode-based photoelectrochemical immunoassay possesses obvious photocurrent response and lower detection limit for ideal sample detection, but it has the inherent imperfection of poor anti-interference capability for real sample detection. Photocathode-based immunoassay can well avoid the intrinsic drawback of photoanode-based immunoassay, but it has low photocurrent response resulting in less good sensitivity. Herein, a promising new cathode photoelectrochemical immunosensing platform integrating photocathode with photoanode was reported for accurate and sensitive detection of biomarkers. In this proposal, prostate-specific antigen (PSA, Ag) was chosen as a model of target analyte to exhibit the analytical performances of this platform. TiO₂/CdS:Mn hybrid structure modified indium–tin oxide (ITO) electrode served as photoanode, whereas CuInS₂ microflowers modified ITO electrode was selected as photocathode. The transducer elements of PSA antibody (Ab) were modified on photocathode to fabricate a label-free cathode immunosensing electrode. The proposed immunosensing platform possesses two distinct advantages simultaneously. First, it has good anti-interference capability for the detection of real biological samples, since the biorecognition events occurred on photocathode. Second, the photoelectrochemical system owns evident photocurrent response and low detection limit for target Ag detection thanks to the introduction of the photoanode. Moreover, the proposed immunosensing platform also exhibits good specificity, reproducibility, and stability, and meanwhile it opens up a new horizon to construct other kinds of photoelectrochemical biosensors.

更新日期：2016-10-19

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