<br>用于内源 HClO 爆发长期连续成像的高度稳定比例探针的从头设计,Analytical Chemistry

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用于内源 HClO 爆发长期连续成像的高度稳定比例探针的从头设计
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-03-03 , DOI: 10.1021/acs.analchem.3c05056
Wenqiang Chen ₁ , Wenju Xu ₁ , Jiayi Xing ₂ , Qixuan Liu ₁ , Jinshuai Wang ₁ , Meijun Meng ₁ , Jiarong Sheng ₁ , Qi Xiao ₁ , Lintao Zeng ₃ , Lei Yang ₂

Affiliation

内源性 HClO 爆发的长期连续成像对于阐明各种生理或病理过程具有重要意义。然而，目前报道的大多数 HClO 探针由于在激光源下的抗光漂白性不足而未能实现这一目标。在此，我们明智地开发了一种高度稳定的比例探针HFTC-HClO 1，它能够长时间连续监测内源性HClO爆发。简而言之，HFTC-HClO 1 的从头开发主要涉及三个主要步骤：（1）设计并合成新型香豆素（HFTC 1-5）；（2）通过染料筛选和细胞成像验证，选择最稳定的支架HFTC 3； (3)基于HFTC 3，构建了3个候选HClO探针，最终选择HFTC-HClO 1，因为其对HClO具有优异的传感性能。此外，HFTC-HClO 1 可以定量测量各种实际样品中的 HClO 水平，且回收率优异（>90.4%），并且还实现了使用 HFTC-HClO 1 涂层测试条对实际样品中 HClO 进行定性分析。此外，还成功证明了HFTC-HClO 1 在长期连续监测细胞内HClO爆发方面的应用。值得注意的是，HFTC-HClO 1 能够可视化类风湿性关节炎小鼠模型中产生的 HClO。

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De Novo Design of a Highly Stable Ratiometric Probe for Long-Term Continuous Imaging of Endogenous HClO Burst

Long-term continuous imaging of endogenous HClO burst is of great importance for the elucidation of various physiological or pathological processes. However, most of the currently reported HClO probes have failed to achieve this goal due to their insufficient photobleaching resistance under a laser source. Herein, a highly stable ratiometric probe, HFTC-HClO 1, which is capable of continuously monitoring endogenous HClO burst over a long period of time, has been judiciously developed. Briefly, the de novo development of HFTC-HClO 1 mainly involved three main steps: (1) novel coumarins (HFTC 1–5) were designed and synthesized; (2) the most stable scaffold, HFTC 3, was selected through dye screening and cell imaging validation; and (3) based on HFTC 3, three candidate HClO probes were constructed, and HFTC-HClO 1 was finally selected due to its superior sensing properties toward HClO. Furthermore, HFTC-HClO 1 can quantitatively measure HClO levels in various real samples with excellent recovery (>90.4%), and the use of HFTC-HClO 1-coated test strips for qualitative analysis of HClO in real samples was also achieved. In addition, the application of HFTC-HClO 1 for long-term continuous monitoring of intracellular HClO burst was successfully demonstrated. Significantly, HFTC-HClO 1 was able to visualize HClO generated in the rheumatoid arthritis mouse model.

更新日期：2024-03-03

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