<br>河水环境中抗生素选择性压力下微塑料生物膜定植对 ARGs 丰度的调节,Journal of Environmental Management

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河水环境中抗生素选择性压力下微塑料生物膜定植对 ARGs 丰度的调节
Journal of Environmental Management ( IF 8.0 ) Pub Date : 2024-02-29 , DOI: 10.1016/j.jenvman.2024.120402
Zhijie Zheng ₁ , Xiaolong Wang ₁ , Wenzhu Zhang ₁ , Lan Wang ₁ , Honghong Lyu ₂ , Jingchun Tang ₁

Affiliation

水生环境中微塑料（MP）生物膜与抗生素抗性基因（ARG）和抗生素的相互作用使微塑料生物膜成为学术界热衷的问题。抗生素选择性压力下生物膜的形成过程和ARGs富集程度以及对微生物群落的影响需要进一步研究。本文研究了环丙沙星（CIP）的选择压力和光照条件对聚氯乙烯（PVC）微塑料生物膜的理化性质、生物量和细胞外聚合物分泌的影响。此外，通过实时定量聚合酶链反应（qPCR）分析了九个ARG的相对拷贝数。在存在 CIP 的情况下，水和微塑料生物膜中的微生物更倾向于携带相关的 ARG（高出 2-3 倍），这对 ARG 富集有贡献。与周围水体（总相对丰度高达0.488）相比，微塑料前生物膜形成过程可能对ARG（总相对丰度高达0.151）的转移和增殖具有抑制作用。然而，在 CIP 胁迫下，微塑料生物膜比周围的水（从 0.488 到 0.386）更好地保持了 ARG 丰度（从 0.151 到 0.149）。因此，微塑料生物膜充当 ARG 丰富的缓冲岛，稳定 ARG 的浓度。此外，高通量分析显示不同条件下生物膜中存在抗生素抗性（）和致病性（）微生物。上述研究加深了我们对生物膜中 ARGs 富集的理解，并为 ARGs、抗生素和微塑料生物膜之间相互作用的生态风险提供了重要见解。

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Regulation of ARGs abundance by biofilm colonization on microplastics under selective pressure of antibiotics in river water environment

Interactions of microplastics (MPs) biofilm with antibiotic resistance genes (ARGs) and antibiotics in aquatic environments have made microplastic biofilm an issue of keen scholarly interest. The process of biofilm formation and the degree of ARGs enrichment in the presence of antibiotic-selective pressure and the impact on the microbial community need to be further investigated. In this paper, the selective pressure of ciprofloxacin (CIP) and illumination conditions were investigated to affect the physicochemical properties, biomass, and extracellular polymer secretion of polyvinyl chloride (PVC) microplastic biofilm. In addition, relative copy numbers of nine ARGs were analyzed by real-time quantitative polymerase chain reaction (qPCR). In the presence of CIP, microorganisms in the water and microplastic biofilm were more inclined to carry associated ARGs (2–3 times higher), which had a contributing effect on ARGs enrichment. The process of pre-microplastic biofilm formation might have an inhibitory effect on ARGs (total relative abundance up to 0.151) transfer and proliferation compared to the surrounding water (total relative abundance up to 0.488). However, in the presence of CIP stress, microplastic biofilm maintained the abundance of ARGs (from 0.151 to 0.149) better compared to the surrounding water (from 0.488 to 0.386). Therefore, microplastic biofilm act as abundance buffer island of ARGs stabilizing the concentration of ARGs. In addition, high-throughput analyses showed the presence of antibiotic-resistant () and pathogenic () microorganisms in biofilm under different conditions. The above research deepens our understanding of ARGs enrichment in biofilm and provides important insights into the ecological risks of interactions between ARGs, antibiotics, and microplastic biofilm.

更新日期：2024-02-29

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