<br>Auxochrome 二甲基二氢吖啶可改善荧光团，实现长时间的活细胞超分辨率成像,Journal of the American Chemical Society

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Auxochrome 二甲基二氢吖啶可改善荧光团，实现长时间的活细胞超分辨率成像
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2024-02-29 , DOI: 10.1021/jacs.3c11823
Xiaojie Ren _{1,

2} , Chao Wang ₃ , Xia Wu ₃ , Mengtao Rong ₄ , Rong Huang ₁ , Qin Liang ₁ , Tianruo Shen ₃ , Hongyan Sun ₂ , Ruilong Zhang ₄ , Zhongping Zhang ₄ , Xiaogang Liu ₃ , Xiangzhi Song ₁ , James W Foley ₅

Affiliation

卓越的光稳定性、最小的光毒性、红移吸收/发射波长、高亮度和扩大的斯托克斯位移是顶级有机荧光团的基本特征，特别是对于活细胞中的持久超分辨率成像（例如，通过受激发射）耗尽（STED）纳米显微镜）。然而，现有的荧光团很少具有所有这些特性。在这项研究中，我们展示了通过引入 9,9-二甲基-9,10-二氢吖啶 (DMA) 作为供电子助色剂同时增强这些参数的通用方法。 DMA 不仅会引起发射波长的红移，还能抑制光氧化反应并防止基于 DMA 的荧光团中三线态的形成，从而大大提高光稳定性并显着降低光毒性。此外，DMA基团增强了荧光团的亮度并扩大了斯托克斯位移。重要的是，连接 DMA 助色剂的“普遍”益处已在各种荧光团中得到体现，包括罗丹明、二氟化硼复合物和香豆素衍生物。由此产生的荧光团成功地实现了细胞器和 HaloTag 标记的膜蛋白的 STED 成像。

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Auxochrome Dimethyl-Dihydroacridine Improves Fluorophores for Prolonged Live-Cell Super-Resolution Imaging

Superior photostability, minimal phototoxicity, red-shifted absorption/emission wavelengths, high brightness, and an enlarged Stokes shift are essential characteristics of top-tier organic fluorophores, particularly for long-lasting super-resolution imaging in live cells (e.g., via stimulated emission depletion (STED) nanoscopy). However, few existing fluorophores possess all of these properties. In this study, we demonstrate a general approach for simultaneously enhancing these parameters through the introduction of 9,9-dimethyl-9,10-dihydroacridine (DMA) as an electron-donating auxochrome. DMA not only induces red shifts in emission wavelengths but also suppresses photooxidative reactions and prevents the formation of triplet states in DMA-based fluorophores, greatly improving photostability and remarkably minimizing phototoxicity. Moreover, the DMA group enhances the fluorophores’ brightness and enlarges the Stokes shift. Importantly, the “universal” benefits of attaching the DMA auxochrome have been exemplified in various fluorophores including rhodamines, difluoride-boron complexes, and coumarin derivatives. The resulting fluorophores successfully enabled the STED imaging of organelles and HaloTag-labeled membrane proteins.

更新日期：2024-02-29

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