Double-Network Hydrogel with Strengthened Mechanical Property for Controllable Release of Antibacterial Peptide,Biomacromolecules

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Double-Network Hydrogel with Strengthened Mechanical Property for Controllable Release of Antibacterial Peptide
Biomacromolecules ( IF 5.5 ) Pub Date : 2024-02-28 , DOI: 10.1021/acs.biomac.3c01290
Tingyuan Tan ₁ , Yangqian Hou ₂ , Yi Zhang ₂ , Biao Wang _{1,

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Affiliation

Developing double-network (DN) hydrogels with high mechanical properties and antibacterial efficacy to combat multidrug-resistant bacterial infections and serve as scaffolds for cell culture still remains an ongoing challenge. In this study, an ion-responsive antibacterial peptide (AMP) (C₁₆-WIIIKKK, termed as IK7) was synergistically combined with a photoresponsive gelatin methacryloyl (GelMA) polymer to fabricate a biocompatible DN hydrogel. The GelMA-IK7 DN hydrogel showed enhanced mechanical properties in contrast to the individual IK7 and GelMA hydrogels and demonstrated substantial antibacterial efficacy. Further investigations revealed that the DN hydrogel effectively inhibited bacterial growth by the controlled and sustained release of the IK7 peptide. In addition, the formation of the DN hydrogel was also found to protect AMP IK7 from rapid degradation by proteinase K. Our findings suggested that the developed GelMA-IK7 DN hydrogel holds great potential for next-generation antibacterial hydrogels for three-dimensional cell culture and tissue regeneration.

中文翻译：

具有增强机械性能的双网络水凝胶，用于抗菌肽的可控释放

开发具有高机械性能和抗菌功效的双网络（DN）水凝胶以对抗多重耐药细菌感染并用作细胞培养的支架仍然是一个持续的挑战。在这项研究中，离子响应抗菌肽（AMP）（C 16-WIIIKKK，称为 IK7）与光响应明胶甲基丙烯酰（GelMA）聚合物协同结合，制备了生物相容性 DN 水凝胶。与单独的 IK7 和 GelMA 水凝胶相比，GelMA-IK7 DN 水凝胶显示出增强的机械性能，并显示出显着的抗菌功效。进一步的研究表明，DN 水凝胶通过控制和持续释放 IK7 肽有效抑制细菌生长。此外，还发现 DN 水凝胶的形成可以保护 AMP IK7 免受蛋白酶 K 的快速降解。我们的研究结果表明，开发的 GelMA-IK7 DN 水凝胶在下一代抗菌水凝胶中具有巨大的潜力，可用于三维细胞培养和组织再生。

更新日期：2024-02-28

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