<br>用于 GTR 应用的蛋壳膜生物材料的制备和优化,Dental Materials

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用于 GTR 应用的蛋壳膜生物材料的制备和优化
Dental Materials ( IF 4.6 ) Pub Date : 2024-02-24 , DOI: 10.1016/j.dental.2024.02.008
Lohitha Kalluri ₁ , Jason A Griggs ₁ , Amol V Janorkar ₁ , Xiaoming Xu ₂ , Ravi Chandran ₃ , Hao Mei ₄ , Kadie P Nobles ₁ , Shan Yang ₅ , Laura Alberto ₁ , Yuanyuan Duan ₁

Affiliation

引导组织再生（GTR）是一种流行的牙周组织再生临床手术。然而，其关键成分屏障膜主要基于胶原蛋白，并且仍然相当昂贵，给患者和医疗保健系统带来经济负担，并对患者的决策产生负面影响。因此，我们的目标是利用天然生物材料可溶性蛋壳膜蛋白（SEP）制备新型仿生 GTR 膜，与胶原蛋白不同，它是经济的，因为它来自食品和家禽业的丰富工业废物。添加添加剂聚合物聚乳酸乙醇酸 (PLGA) 和生物陶瓷纳米羟基磷灰石 (HAp) 以改善其机械和生物性能。对于这种隔膜制备，我们首先利用田口正交阵列设计筛选了影响其力学性能的重要因素，并利用响应面法进一步优化了重要因素。此外，使用 SEM、EDAX 和 ATR-FTIR 对该膜进行了表征，并测试了人牙周膜成纤维细胞 (HPLF) 的增殖活性。使用响应面法进行优化预测，在膜成分为 8.9 wt% PLGA、7.2 wt% SEP 和 2 wt% HAp 时，可以获得 3.1 MPa 的最大拉伸强度和 39.9 MPa 的模量。与组织培养聚苯乙烯和商业胶原膜相比，在静态收集器上电纺的优化 PLGA/SEP/HAp 膜样本显示出更高的 HPLF 增殖活性。从观察到的结果，我们可以得出结论，基于 SEP 的纳米纤维 GTR 膜可能是基于胶原蛋白的商业 GTR 膜产品的一种有前途、环境友好且具有成本效益的替代品。

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Preparation and optimization of an eggshell membrane-based biomaterial for GTR applications

Guided Tissue Regeneration (GTR) is a popular clinical procedure for periodontal tissue regeneration. However, its key component, the barrier membrane, is largely collagen-based and is still quite expensive, posing a financial burden to the patients as well as healthcare systems and negatively impacting the patient’s decision-making. Thus, our aim is to prepare a novel biomimetic GTR membrane utilizing a natural biomaterial, soluble eggshell membrane protein (SEP), which is economical as it comes from an abundant industrial waste from food and poultry industries, unlike collagen. Additive polymer, poly (lactic-co-glycolic acid) (PLGA), and a bioceramic, nano-hydroxyapatite (HAp), were added to improve its mechanical and biological properties. For this barrier membrane preparation, we initially screened the significant factors affecting its mechanical properties using Taguchi orthogonal array design and further optimized the significant factors using response surface methodology. Furthermore, this membrane was characterized using SEM, EDAX, and ATR-FTIR, and tested for proliferation activity of human periodontal ligament fibroblasts (HPLFs). Optimization using response surface methodology predicted that the maximal tensile strength of 3.1 MPa and modulus of 39.9 MPa could be obtained at membrane composition of 8.9 wt% PLGA, 7.2 wt% of SEP, and 2 wt% HAp. Optimized PLGA/SEP/HAp membrane specimens that were electrospun on a static collector showed higher proliferation activity of HPLFs compared to tissue culture polystyrene and a commercial collagen membrane. From the results observed, we can conclude that SEP-based nanofibrous GTR membrane could be a promising, environment-friendly, and cost-effective alternative for commercial collagen-based GTR membrane products.

更新日期：2024-02-24

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