Biophysical Characterization and Cryopreservation of Mammalian Cells Using Ionic Liquids,The Journal of Physical Chemistry B

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Biophysical Characterization and Cryopreservation of Mammalian Cells Using Ionic Liquids
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2024-02-28 , DOI: 10.1021/acs.jpcb.3c06797
Miyah N Awad ₁ , Stuart J Brown ₁ , Amanda N Abraham _{1,

2} , Dilek Sezer ₁ , Qi Han ₁ , Xiaoying Wang _{3,

4} , Tu C Le ₃ , Aaron Elbourne ₁ , Gary Bryant ₁ , Tamar L Greaves ₁ , Saffron J Bryant ₁

Affiliation

Ionic liquids (ILs) are a diverse class of solvents which can be selected for task-specific properties, making them attractive alternatives to traditional solvents. To tailor ILs for specific biological applications, it is necessary to understand the structure–property relationships of ILs and their interactions with cells. Here, a selection of carboxylate anion-based ILs were investigated as cryoprotectants, which are compounds added to cells before freezing to mitigate lethal freezing damage. The cytotoxicity, cell permeability, thermal behavior, and cryoprotective efficacy of the ILs were assessed with two model mammalian cell lines. We found that the biophysical interactions, including permeability of the ILs, were influenced by considering the IL pair together, rather than as single species acting independently. All of the ILs tested had high cytotoxicity, but ethylammonium acetate demonstrated good cryoprotective efficacy for both cell types tested. These results demonstrate that despite toxicity, ILs may be suitable for certain biological applications. It also demonstrates that more research is required to understand the contribution of ion pairs to structure–property relationships and that knowing the behavior of a single ionic species will not necessarily predict its behavior as part of an IL.

中文翻译：

使用离子液体对哺乳动物细胞进行生物物理表征和冷冻保存

离子液体 (IL) 是一类多种溶剂，可根据特定任务的特性进行选择，使其成为传统溶剂的有吸引力的替代品。为了针对特定的生物应用定制IL，有必要了解IL的结构-性质关系及其与细胞的相互作用。在这里，我们研究了一系列基于羧酸根阴离子的离子液体作为冷冻保护剂，这些化合物是在冷冻前添加到细胞中以减轻致命的冷冻损伤的化合物。使用两种模型哺乳动物细胞系评估了 IL 的细胞毒性、细胞通透性、热行为和冷冻保护功效。我们发现生物物理相互作用，包括 IL 的渗透性，受到 IL 对一起考虑的影响，而不是作为单个物种独立作用。所有测试的 IL 均具有高细胞毒性，但醋酸乙铵对两种测试的细胞类型均表现出良好的冷冻保护功效。这些结果表明，尽管有毒性，离子液体可能适合某些生物应用。它还表明，需要更多的研究来了解离子对对结构-性质关系的贡献，并且了解单个离子种类的行为不一定能预测其作为离子液体一部分的行为。

更新日期：2024-02-28

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