The prevalence of food allergies has grown dramatically over the past decade. Recently, studies have shown the potential of marine substances to alleviate food allergies. We utilized a rat basophilic leukemia (RBL)-2H3 model to evaluate the antiallergic effects of alternariol monomethyl ether (AME) extracted from marine fungi Alternaria sp. Our results showed that AME attenuated food allergy symptoms in mice and reduced histamine release in serum. The population of mast cells in the spleen and mesenteric lymph nodes was considerably reduced. Moreover, in vitro assays also revealed that AME inhibited the release of β-hexosaminidase and histamine. Transcriptomic analysis uncovered that AME regulated gene expression associated with mast cells. Additionally, Western blotting demonstrated that AME suppressed mast cell activation by modulating MAPK and NF-κB signaling pathways. Taken together, these findings provide a theoretical basis for the potential antiallergic use of marine-derived compounds in the development of functional foods.

中文翻译：

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海洋来源的链格孢醇单甲醚通过抑制肥大细胞的 MAPK 和 NF-κB 信号通路减轻卵清蛋白引起的食物过敏

在过去的十年中，食物过敏的患病率急剧上升。最近，研究表明海洋物质具有缓解食物过敏的潜力。我们利用大鼠嗜碱性白血病 (RBL)-2H3 模型来评估从海洋真菌链格孢 ( Alternaria sp ) 中提取的链格孢醇单甲醚 (AME) 的抗过敏作用。我们的结果表明，AME 减轻了小鼠的食物过敏症状，并减少了血清中组胺的释放。脾脏和肠系膜淋巴结中的肥大细胞数量显着减少。此外，体外试验还表明，AME 抑制 β-己糖胺酶和组胺的释放。转录组分析发现 AME 调节与肥大细胞相关的基因表达。此外，蛋白质印迹表明 AME 通过调节 MAPK 和 NF-κB 信号通路抑制肥大细胞活化。总而言之，这些发现为海洋源性化合物在功能性食品开发中潜在的抗过敏用途提供了理论基础。