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Study on the Efficacy and Potential Mechanism of Topical Shen Bai Hair Growing Decoction against Androgenetic Alopecia Based on Ultrahigh Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry and RNA-seq
ACS Omega ( IF 3.7 ) Pub Date : 2024-02-25 , DOI: 10.1021/acsomega.3c09648 Mingxi Li 1, 2, 3 , Xiujun Zhang 4 , Yan Wang 1, 2, 3 , Beibei Xiang 3 , Zhaoyi Liu 3 , Wenwen Zhang 3 , Xuanming Liu 3 , Ruoxi Guo 5
ACS Omega ( IF 3.7 ) Pub Date : 2024-02-25 , DOI: 10.1021/acsomega.3c09648 Mingxi Li 1, 2, 3 , Xiujun Zhang 4 , Yan Wang 1, 2, 3 , Beibei Xiang 3 , Zhaoyi Liu 3 , Wenwen Zhang 3 , Xuanming Liu 3 , Ruoxi Guo 5
Affiliation
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Androgenetic alopecia (AGA) is a major problem that can happen to people of all ages, leading to psychological problems, such as anxiety and depression. Topical Shen Bai hair growing decoction (TSBHGD) is based on the pathogenesis of AGA, combined with Traditional Chinese Medicine theory, improved by the Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital according to its clinical treatment experience. This study was designed to demonstrate the therapeutic efficacy of TSBHGD against AGA, analyze the chemical components of TSBHGD as well as the skin-retained and blood-retained components in mice after topical administration of TSBHGD, and clarify the mechanism of its therapeutic efficacy. It was demonstrated that TSBHGD could suppress TNF-α and IL-6 levels and improve pathological phenomena such as hair loss, reduced follicle density, and dermal thickness caused by testosterone solution. Totally 35 components were identified in TSBHGD extracts, 12 skin-retained components were identified in drug-containing skin, and 7 blood-retained components were identified in drug-containing plasma, according to ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry. Transcriptomic sequencing revealed that some of the genes in AGA mice had altered expression patterns, which could be reversed by TSBHGD. Through network pharmacology analysis, it was found that TSBHGD mainly regulated eight signaling pathways, among which the apoptosis signaling pathway ranked first with a significance of 0.00149. Finally, both Bcl-2 and Caspase family proteins in the apoptosis signaling pathway were examined by Western blot. It was confirmed that TSBHGD could inhibit the apoptosis level in AGA mice’s skin tissue to exert an anti-AGA effect. This will facilitate the development of new-generation herbal compound formulas with precise efficacy and provide novel ideas for AGA therapy.
中文翻译：
基于超高效液相色谱四极杆飞行时间质谱和RNA-seq的外用参白生发汤治疗雄激素性脱发的功效及潜在机制研究
雄激素性脱发（AGA）是所有年龄段的人都可能发生的一个主要问题，会导致焦虑和抑郁等心理问题。外用参白生发汤（TSBHGD）是根据AGA的发病机理，结合中医理论，由天津中医药研究院附属医院根据临床治疗经验改进而成。本研究旨在论证TSBHGD对AGA的治疗功效，分析TSBHGD的化学成分以及局部给予TSBHGD后小鼠皮肤保留和血液保留的成分，并阐明其治疗作用的机制。结果表明，TSBHGD可抑制TNF-α和IL-6水平，改善睾酮溶液引起的脱发、毛囊密度降低、真皮厚度等病理现象。超高效液相色谱四极杆飞行时间质谱法检测出TSBHGD提取物中35种成分，含药皮肤中检出12种皮肤滞留成分，含药血浆中检出7种血液滞留成分。转录组测序显示，AGA 小鼠中的一些基因已经改变了表达模式，而 TSBHGD 可以逆转这种模式。通过网络药理学分析发现，TSBHGD主要调控8条信号通路，其中凋亡信号通路位居首位，显着性为0.00149。最后通过Western blot检测凋亡信号通路中的Bcl-2和Caspase家族蛋白。证实TSBHGD可以抑制AGA小鼠皮肤组织的凋亡水平，从而发挥抗AGA作用。 这将有利于开发新一代功效精准的中药复方，为AGA治疗提供新思路。
更新日期：2024-02-25
中文翻译：
基于超高效液相色谱四极杆飞行时间质谱和RNA-seq的外用参白生发汤治疗雄激素性脱发的功效及潜在机制研究
雄激素性脱发（AGA）是所有年龄段的人都可能发生的一个主要问题，会导致焦虑和抑郁等心理问题。外用参白生发汤（TSBHGD）是根据AGA的发病机理，结合中医理论，由天津中医药研究院附属医院根据临床治疗经验改进而成。本研究旨在论证TSBHGD对AGA的治疗功效，分析TSBHGD的化学成分以及局部给予TSBHGD后小鼠皮肤保留和血液保留的成分，并阐明其治疗作用的机制。结果表明，TSBHGD可抑制TNF-α和IL-6水平，改善睾酮溶液引起的脱发、毛囊密度降低、真皮厚度等病理现象。超高效液相色谱四极杆飞行时间质谱法检测出TSBHGD提取物中35种成分，含药皮肤中检出12种皮肤滞留成分，含药血浆中检出7种血液滞留成分。转录组测序显示，AGA 小鼠中的一些基因已经改变了表达模式，而 TSBHGD 可以逆转这种模式。通过网络药理学分析发现，TSBHGD主要调控8条信号通路，其中凋亡信号通路位居首位，显着性为0.00149。最后通过Western blot检测凋亡信号通路中的Bcl-2和Caspase家族蛋白。证实TSBHGD可以抑制AGA小鼠皮肤组织的凋亡水平，从而发挥抗AGA作用。 这将有利于开发新一代功效精准的中药复方，为AGA治疗提供新思路。
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