In this study, the effects of CO₂ addition on the growth performance and biochemical composition of the green microalga Tetradesmus obliquus cultured in a hybrid algal production system (HAPS) were investigated. The HAPS combines the characteristics of tubular photobioreactors (towards a better carbon dioxide dissolution coefficient) with thin-layer cascade system (with a higher surface-to-volume ratio). Experimental batches were conducted with and without CO₂ addition, and evaluated in terms of productivity and biomass characteristics (elemental composition, protein and lipid contents, pigments and fatty acids profiles). CO₂ enrichment positively influenced productivity, and proteins, lipids, pigments and unsaturated fatty acids contents in biomass. The HAPS herein presented contributes to the optimization of microalgae cultures in open systems, since it allows, with a simple adaptation—a transit of the cultivation through a tubular portion where injection and dissolution of CO₂ is efficient—to obtain in TLC systems, greater productivity and better-quality biomass.

在本研究中，研究了添加CO ₂对在混合藻类生产系统（HAPS）中培养的绿色微藻Tetradesmus obliquus的生长性能和生化组成的影响。 HAPS 结合了管式光生物反应器的特性（更好的二氧化碳溶解系数）和薄层级联系统（具有更高的表面积与体积比）。在添加和不添加CO ₂的情况下进行实验批次，并评估生产率和生物质特征（元素组成、蛋白质和脂质含量、色素和脂肪酸谱）。 CO ₂富集对生产力以及生物质中的蛋白质、脂质、色素和不饱和脂肪酸含量产生积极影响。本文提出的 HAPS 有助于优化开放系统中的微藻培养，因为它允许通过简单的调整（通过管状部分进行培养，其中 CO ₂的注射和溶解是有效的）在 TLC 系统中获得更大的效果。生产力和更好质量的生物质。