Synucleinopathies are a group of neurodegenerative diseases (NDs) associated with cerebral accumulation of α-synuclein (αSyn) misfolded aggregates. At this time, there is no effective treatment to stop or slow down disease progression, which in part is due to the lack of an early and objective biochemical diagnosis. In the past 5 years, the seed amplification technology has emerged for highly sensitive identification of these diseases, even at the preclinical stage of the illness. Much research has been done in multiple laboratories to validate the efficacy and reproducibility of this assay. This article provides a comprehensive review of this technology, including its conceptual basis and its multiple applications for disease diagnosis, as well for understanding of the disease biology and therapeutic development.

中文翻译：

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用于帕金森病和相关突触核蛋白病的 α-突触核蛋白种子扩增技术


突触核蛋白病是一组与大脑中 α-突触核蛋白 (αSyn) 错误折叠聚集体积累相关的神经退行性疾病 (ND)。目前，尚无有效的治疗方法来阻止或减缓疾病进展，部分原因是缺乏早期客观的生化诊断。在过去的 5 年里，种子扩增技术的出现可以对这些疾病进行高度灵敏的识别，即使是在疾病的临床前阶段。多个实验室已进行了大量研究来验证该测定的有效性和可重复性。本文对该技术进行了全面的回顾，包括其概念基础及其在疾病诊断方面的多种应用，以及对疾病生物学和治疗发展的理解。