<br>用于检测细胞和水溶性系统中 ClO− 的荧光生物质平台,Applied Spectroscopy

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用于检测细胞和水溶性系统中 ClO− 的荧光生物质平台
Applied Spectroscopy ( IF 2.2 ) Pub Date : 2024-01-09 , DOI: 10.1177/00037028231219524
Jing Nie _{1,

2} , Xinxue Tang _{2,

3} , Jianhua Wei ₂ , Zebiao Li ₃ , Binbin Zhou ₄ , Xi Dai _{2,

5} , Wenli Wu ₆ , Zhonghai Ni ₂

Affiliation

过量次氯酸衍生物离子形式（ClO - ）可能导致多种疾病，如动脉硬化、DNA损伤和心血管疾病。迫切需要开发一种高灵敏度传感器来可靠检测 ClO -在细胞和水溶性系统中。在这项工作中，将羟基引入化合物2-amino-3-(((E)-4-(2-(2-(2-羟基乙氧基)乙基)-1,3-dioxo-2,3 -二氢-1H-苯并[de]异喹啉-6-基)亚苯亚基)氨基)马来腈(NDC)以增加其在水中的溶解度，同时腙单元被设计为“off-”的特异性识别基团。 ClO 荧光探针- 。 NDC 探针对 ClO 具有高选择性、高灵敏度、抗干扰性和低检测限 (67 nM) - 。 ClO的识别机制-断裂 C=N 键并形成荧光化合物 4-(2-(2-(2-羟基乙氧基)乙基)-1,3-二氧代-2,3-二氢-1H-苯并[去]异喹啉-6-基) 苯甲醛 (ND-3) 已通过飞行时间质谱法得到证实。 NDC探头在水样实际测试中表现出良好的性能，可设计作为快速、便捷检测ClO的试纸。 -范围从 0 到 1 μM。此外，内源性和外源性 ClO 的成功成像证明了实际应用-在海拉细胞中。我们基于荧光生物质的平台为 ClO 的重复性、灵敏度和选择性检测提供了巨大的可能性-在细胞和水溶性系统中。

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Fluorescent Biomass-Based Platform for Detection of ClO− in Cells and Water-Soluble Systems

The generation and presence of excessive hypochlorous acid derivative ionic form (ClO−) could cause various diseases, such as arteriosclerosis, DNA damage, and cardiovascular illness. It is a critical need to develop a highly sensitive sensor for reliable detection of ClO− in cells and water-soluble systems. In this work, a hydroxyl group has been introduced into the compound 2-amino-3-(((E)-4-(2-(2-(2-hydroxyethoxy)ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinolin-6-yl)benzylidene)amino)maleonitrile (NDC) to increase its solubility in water, at the same time, the hydrazone unit was designed as a specific recognition group for the “off–on” fluorescence probe of ClO−. The probe NDC presents high selectivity, sensitivity, anti-interference, and low detection limit (67 nM) for ClO−. The recognition mechanism that ClO− breaks the C=N bond and forms the fluorescent compound 4-(2-(2-(2-hydroxyethoxy)ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinolin-6-yl)benzaldehyde (ND-3) has been confirmed by time-of-flight mass spectrometry. The probe NDC presents a good performance in the actual test of water samples and can be designed as the test papers for the quick and convenient detection of ClO− range from 0 to 1 μM. Moreover, the practical application was demonstrated by the successful imaging of endogenous and exogenous ClO− in HeLa cells. Our fluorescent biomass-based platform opens vast possibilities for repeatability, sensitivity, and selectivity detection of ClO− in cells and water-soluble systems.

更新日期：2024-01-09

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