Application of CRISPR/Cas13a-based biosensors in serum marker detection,Analytical Methods

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Application of CRISPR/Cas13a-based biosensors in serum marker detection
Analytical Methods ( IF 2.7 ) Pub Date : 2024-02-22 , DOI: 10.1039/d3ay01927f
Wei He ₁ , Xiyu Liu ₁ , Jintong Na ₁ , Huimin Bian ₁ , Liping Zhong ₁ , Guiyin Li _{1,

2}

Affiliation

The detection of serum markers is important for the early diagnosis and monitoring of diseases, but conventional detection methods have the problem of low specificity or sensitivity. CRISPR/Cas13a-based biosensors have the characteristics of simple detection methods and high sensitivity, which have a certain potential to solve the problems of conventional detection. This paper focuses on the research progress of CRISPR/Cas13a-based biosensors in serum marker detection, introduces the principles and applications of fluorescence, electrochemistry, colorimetric, and other biosensors based on CRISPR/Cas13a in the detection of serum markers, compares and analyzes the differences between the above CRISPR/Cas13a-based biosensors, and looks forward to the future development direction of CRISPR/Cas13a-based biosensors.

中文翻译：

基于CRISPR/Cas13a的生物传感器在血清标志物检测中的应用

血清标志物的检测对于疾病的早期诊断和监测具有重要意义，但常规检测方法存在特异性或敏感性较低的问题。基于CRISPR/Cas13a的生物传感器具有检测方法简单、灵敏度高的特点，有一定潜力解决常规检测的问题。本文重点介绍了基于CRISPR/Cas13a的生物传感器在血清标志物检测中的研究进展，介绍了基于CRISPR/Cas13a的荧光、电化学、比色等生物传感器在血清标志物检测中的原理和应用，并对现有技术进行了比较分析。比较了上述基于CRISPR/Cas13a的生物传感器之间的差异，并展望了基于CRISPR/Cas13a的生物传感器的未来发展方向。

更新日期：2024-02-22

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