Tissue repair and fibrosis involve the dynamic remodeling of collagen, and accurate detection of these sites is of utmost importance. Here, we use a collagen peptide sensor () to visualize collagen formation and remodeling during wound healing in mice and humans. We show that the probe binds selectively to sites of collagen formation and remodeling at different stages of healing. Compared to conventional methods, the peptide sensor localizes preferentially to areas of collagen synthesis and remodeling at the wound edge and not in matured fibrillar collagen. We also demonstrate its applicability for wound imaging and for discerning differential remodeling in wounds of transgenic mice with altered collagen dynamics. Our findings show the value of as a diagnostic tool to rapidly identify the sites of matrix remodeling in tissue sections, which will aid in the conception of new therapeutic strategies for fibrotic disorders and defective tissue repair.

中文翻译：

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赖氨酰氧化酶响应性胶原蛋白肽阐明伤口愈合中的胶原蛋白重塑


组织修复和纤维化涉及胶原蛋白的动态重塑，准确检测这些部位至关重要。在这里，我们使用胶原蛋白肽传感器 () 来可视化小鼠和人类伤口愈合过程中胶原蛋白的形成和重塑。我们表明，探针在愈合的不同阶段选择性地结合到胶原蛋白形成和重塑的位点。与传统方法相比，肽传感器优先定位于伤口边缘的胶原蛋白合成和重塑区域，而不是成熟的纤维状胶原蛋白。我们还证明了它在伤口成像和辨别胶原动力学改变的转基因小鼠伤口的差异重塑方面的适用性。我们的研究结果显示了作为快速识别组织切片中基质重塑位点的诊断工具的价值，这将有助于构思纤维化疾病和有缺陷的组织修复的新治疗策略。