<br>诺氟沙星和聚苯乙烯纳米颗粒对马蹄蟹幼年氧化应激和肠道健康的联合影响,Journal of Hazardous Materials

当前位置： X-MOL 学术 › J. Hazard. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

诺氟沙星和聚苯乙烯纳米颗粒对马蹄蟹幼年氧化应激和肠道健康的联合影响
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2024-02-15 , DOI: 10.1016/j.jhazmat.2024.133801
Meilian Huang ₁ , Yuanxiong Ma ₁ , Jin Qian ₁ , Inna M Sokolova ₂ , Caoqi Zhang ₁ , Khor Waiho ₃ , James Kar Hei Fang ₄ , Xiaowan Ma ₅ , Youji Wang ₁ , Menghong Hu ₁

Affiliation

包括抗生素和纳米塑料在内的人为污染物污染导致海洋环境逐渐恶化，从而威胁到鲎等濒危物种。我们评估了抗生素（诺氟沙星，0、0.5、5 μg/L）和聚苯乙烯纳米颗粒（10 颗粒/L）在使用组织氧化还原状态、蜕皮和肠道微生物群的生物标志物中的潜在毒性机制。暴露于单一和混合污染物导致短期（7 天）暴露期间氧化还原平衡紊乱，表现为脂质过氧化产物丙二醛（MDA）水平升高。长时间（14-21 天）暴露后，观察到抗氧化剂（过氧化氢酶和谷胱甘肽，但不是超氧化物歧化酶）的代偿性上调，并且在大多数实验暴露中 MDA 水平恢复到基线水平。蜕皮相关基因（蜕皮激素受体、视黄酸 X α 受体和钙调蛋白 A）和分子伴侣（同源热休克蛋白 70）的转录水平显示对聚苯乙烯纳米颗粒和诺氟沙星的反应证据较弱。肠道微生物群因暴露于诺氟沙星和聚苯乙烯纳米颗粒而发生改变，表现为拟杆菌门的相对丰度升高。在功能水平上，在与遗传信息处理、代谢、有机体系统和环境信息处理相关的多个肠道微生物组途径中发现了诺氟沙星和聚苯乙烯纳米颗粒抑制的证据。需要未来的研究来评估诺氟沙星和聚苯乙烯纳米颗粒引起的微生物组菌群失调的生理和健康后果，并协助对马蹄蟹野生种群中这些污染物的环境风险评估。

"点击查看英文标题和摘要"

Combined effects of norfloxacin and polystyrene nanoparticles on the oxidative stress and gut health of the juvenile horseshoe crab Tachypleus tridentatus

Pollution with anthropogenic contaminants including antibiotics and nanoplastics leads to gradual deterioration of the marine environment, which threatens endangered species such as the horseshoe crab . We assessed the potential toxic mechanisms of an antibiotic (norfloxacin, 0, 0.5, 5 μg/L) and polystyrene nanoparticles (10 particles/L) in using biomarkers of tissue redox status, molting, and gut microbiota. Exposure to single and combined pollutants led to disturbance of redox balance during short-term (7 days) exposure indicated by elevated level of a lipid peroxidation product, malondialdehyde (MDA). After prolonged (14–21 days) exposure, compensatory upregulation of antioxidants (catalase and glutathione but not superoxide dismutase) was observed, and MDA levels returned to the baseline in most experimental exposures. Transcript levels of molting-related genes (ecdysone receptor, retinoic acid X alpha receptor and calmodulin A) and a molecular chaperone (cognate heat shock protein 70) showed weak evidence of response to polystyrene nanoparticles and norfloxacin. The gut microbiota was altered by exposures to norfloxacin and polystyrene nanoparticles shown by elevated relative abundance of Bacteroidetes. At the functional level, evidence of suppression by norfloxacin and polystyrene nanoparticles was found in multiple intestinal microbiome pathways related to the genetic information processing, metabolism, organismal systems, and environmental information processing. Future studies are needed to assess the physiological and health consequences of microbiome dysbiosis caused by norfloxacin and polystyrene nanoparticles and assist the environmental risk assessment of these pollutants in the wild populations of the horseshoe crabs.

更新日期：2024-02-15

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南