Phthalates are synthetic plasticizers present in the daily lives of humans, as part of the composition of different products, such as food packaging, water bottles, and toys. These compounds can migrate from plastic materials to the environment changing biological systems. Although diisopentyl phthalate (DiPeP) is largely used in Brazil, there is a lack of information on the possible toxic effects of this compound. This research aims to evaluate the toxicity of DiPeP in the Vero renal cells. These cells were exposed to the 1–1000 μM of DiPeP for 24 and 72 h and subsequently, the cytotoxicity, apoptosis and necrosis-inducing potential, and antioxidant system (SOD, GPx, and GST) were investigated. DiPeP neither caused cytotoxicity nor altered SOD and GPx activity, although GST has been increased at 100 or 1 μM (24 and 72 h, respectively). However, cell death by apoptosis and necrosis was observed. These results indicate that DiPeP caused cell death by a non-oxidative stress-mediated mechanism, which shows the relevance of investigate other process in further researches.

中文翻译：

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接触增塑剂邻苯二甲酸二异戊酯会导致 Vero 细胞系死亡


邻苯二甲酸盐是人类日常生活中存在的合成增塑剂，是食品包装、水瓶和玩具等不同产品成分的一部分。这些化合物可以从塑料材料迁移到改变环境的生物系统。尽管邻苯二甲酸二异戊酯 (DiPeP) 在巴西大量使用，但缺乏有关该化合物可能产生的毒性作用的信息。本研究旨在评估 DiPeP 对 Vero 肾细胞的毒性。将这些细胞暴露于 1-1000 μM DiPeP 24 和 72 小时，随后研究细胞毒性、细胞凋亡和坏死诱导潜力以及抗氧化系统（SOD、GPx 和 GST）。尽管 GST 增加至 100 或 1 μM（分别为 24 小时和 72 小时），但 DiPeP 既不会引起细胞毒性，也不会改变 SOD 和 GPx 活性。然而，观察到细胞凋亡和坏死导致的死亡。这些结果表明 DiPeP 通过非氧化应激介导的机制引起细胞死亡，这表明在进一步研究中研究其他过程的相关性。