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L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2024-02-09 , DOI: 10.1016/j.anireprosci.2024.107429 Sameh A Abdelnour 1 , Wael A Khalil 2 , Norhan E Khalifa 3 , Fatma Mohamed Ameen Khalil 4 , Mahmoud A E Hassan 5
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2024-02-09 , DOI: 10.1016/j.anireprosci.2024.107429 Sameh A Abdelnour 1 , Wael A Khalil 2 , Norhan E Khalifa 3 , Fatma Mohamed Ameen Khalil 4 , Mahmoud A E Hassan 5
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Sperm cryopreservation technology significantly contributes to the safeguarding of genetic resources, particularly for endangered species, and supports the use of artificial insemination in domestic animals. Therefore, cryopreservation can negatively affect sperm health and function leading to reduce the freezing ability and fertility potential. Therefore, it is essential to prioritize the improvement of cryotolerance in cryopreserved sperm to enhance reproductive efficiency and ensure sustainability in livestock herds. The main reason for sperm dysfunction after thawing may be related to the excessive amount of oxidative stress (OS) produced during cryopreservation. Scientists have different ways for counteracting this OS including the use of plant extracts, enzymes, minerals, anti-freezing proteins, and amino acids. Recently, one such amino acid is L-proline (LP), which has multiple roles such as osmotic and OS defense, nitrogen, and carbon metabolism, as well as cell survival and signaling. LP has been found in seminal plasma and has recently been added to the freezing extender to improve the various post-thaw parameters of sperm. This improvement is related to the ability of LP to reduce the OS, sustain the plasma membrane and to act as an osmoregulatory agent. Moreover, LP can suppress cell apoptosis by modulating intracellular redox in sperm. This review addresses the ongoing research on the addition of L-proline as an osmoregulatory agent in freezing extenders to increase the cryotolerance of animal spermatozoa to freeze-thaw.
中文翻译：
L-脯氨酸：提高动物低温保存精子的低温耐受性和受精能力的有前途的工具
精子冷冻保存技术极大地有助于保护遗传资源，特别是濒危物种的遗传资源，并支持在家畜中使用人工授精。因此，冷冻保存会对精子的健康和功能产生负面影响，导致冷冻能力和生育潜力降低。因此，有必要优先改善冷冻精子的低温耐受性，以提高繁殖效率并确保畜群的可持续性。精子解冻后功能障碍的主要原因可能与冷冻保存过程中产生过多的氧化应激（OS）有关。科学家们有不同的方法来对抗这种操作系统，包括使用植物提取物、酶、矿物质、抗冻蛋白和氨基酸。最近，此类氨基酸之一是 L-脯氨酸 (LP)，它具有多种作用，例如渗透和 OS 防御、氮和碳代谢以及细胞存活和信号传导。 LP 在精浆中被发现，最近被添加到冷冻稀释剂中以改善精子解冻后的各种参数。这种改善与 LP 降低 OS、维持质膜和充当渗透调节剂的能力有关。此外，LP可以通过调节精子的细胞内氧化还原来抑制细胞凋亡。本综述讨论了正在进行的研究，即在冷冻增量剂中添加 L-脯氨酸作为渗透调节剂，以提高动物精子对冻融的低温耐受性。
更新日期：2024-02-09
中文翻译：
L-脯氨酸：提高动物低温保存精子的低温耐受性和受精能力的有前途的工具
精子冷冻保存技术极大地有助于保护遗传资源，特别是濒危物种的遗传资源，并支持在家畜中使用人工授精。因此，冷冻保存会对精子的健康和功能产生负面影响，导致冷冻能力和生育潜力降低。因此，有必要优先改善冷冻精子的低温耐受性，以提高繁殖效率并确保畜群的可持续性。精子解冻后功能障碍的主要原因可能与冷冻保存过程中产生过多的氧化应激（OS）有关。科学家们有不同的方法来对抗这种操作系统，包括使用植物提取物、酶、矿物质、抗冻蛋白和氨基酸。最近，此类氨基酸之一是 L-脯氨酸 (LP)，它具有多种作用，例如渗透和 OS 防御、氮和碳代谢以及细胞存活和信号传导。 LP 在精浆中被发现，最近被添加到冷冻稀释剂中以改善精子解冻后的各种参数。这种改善与 LP 降低 OS、维持质膜和充当渗透调节剂的能力有关。此外，LP可以通过调节精子的细胞内氧化还原来抑制细胞凋亡。本综述讨论了正在进行的研究，即在冷冻增量剂中添加 L-脯氨酸作为渗透调节剂，以提高动物精子对冻融的低温耐受性。
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