Applying extracts from plants is considered a safe approach in biomedicine and bio-nanotechnology. The present report is considered the first study that evaluated the seeds of Lasiurus scindicus and Panicum turgidum as biogenic agents in the synthesis of silver nanoparticles (AgNPs) which had bioactivity against cancer cells and bacteria. Assessment of NPs activity against varied cell lines (colorectal cancer HCT116 and breast cancer MDA MBA 231 and MCF 10A used as control) was performed beside the antibacterial efficiency. Different techniques (DLS, TEM, EDX and FTIR) were applied to characterize the biosynthesized AgNPs. The phytochemicals from both L. scindicus and Panicum turgidum were identified by GC–MS analysis. Spherical monodisperse NPs at average diameters of 149.6 and 100.4 nm were obtained from seed extract of L. scindicus (L-AgNPs) and P. turgidum, (P-AgNPs) respectively. A strong absorption peak at 3 keV is observed by the EDX spectrum in the tested NPs. Our study provided effective NPs in mitigating the tested cell lines and the lowest IC₅₀ were 7.8 and 10.30 for MDA MB231 treated by L-AgNPs and P-AgNPs, respectively. Both fabricated NPs might differentially target the MDA MB231 cells compared to HCT116 and MCF10A. Ultrastructural changes and damage for the NPs-treated MDA MB231 cells were studied using TEM and LSM analysis. Antibacterial activity was also observed. About 200 compounds were identified in L. scindicus and P. turgidum by GC–MS analysis might be responsible for the NPs reduction and capping abilities. Efficient NPs against cancer cells and microbes were obtained, however large-scale screening is needed to validate our findings.

应用植物提取物被认为是生物医学和生物纳米技术中的安全方法。本报告被认为是第一项评估Lasiurus scindicus和Panicum turgidum种子作为生物制剂合成银纳米颗粒 (AgNP) 的研究，银纳米颗粒具有抗癌细胞和细菌的生物活性。除了抗菌效率之外，还对不同细胞系（结肠直肠癌 HCT116 和乳腺癌 MDA MBA 231 和 MCF 10A 作为对照）的活性进行了评估。应用不同的技术（DLS、TEM、EDX 和 FTIR）来表征生物合成的 AgNP。通过 GC-MS 分析鉴定了L. scindicus和Panicum turgidum中的植物化学物质。从L. scindicus (L-AgNPs) 和P. turgidum (P-AgNPs) 的种子提取物中分别获得平均直径为 149.6 和 100.4 nm 的球形单分散纳米颗粒。通过 EDX 光谱在测试的 NP 中观察到 3 keV 处的强吸收峰。我们的研究提供了有效缓解测试细胞系的 NP，经 L-AgNP 和 P-AgNP 处理的 MDA MB231 的最低 IC ₅₀分别为 7.8 和 10.30。与 HCT116 和 MCF10A 相比，两种制造的 NP 可能以不同的方式靶向 MDA MB231 细胞。使用 TEM 和 LSM 分析研究了 NP 处理的 MDA MB231 细胞的超微结构变化和损伤。还观察到抗菌活性。通过 GC-MS 分析，在L. scindicus和P. turgidum中鉴定出约 200 种化合物可能与 NP 还原和封端能力有关。 获得了针对癌细胞和微生物的有效纳米粒子，但需要大规模筛选来验证我们的发现。