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Molecular Mechanism Underlying Pathogenicity Inhibition by Chitosan in Cochliobolus heterostrophus
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-02-16 , DOI: 10.1021/acs.jafc.3c07968 Huilin Yu 1 , Longhao Su 1 , Wantong Jia 1 , Mengjiao Jia 1 , Hongyu Pan 1 , Xianghui Zhang 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-02-16 , DOI: 10.1021/acs.jafc.3c07968 Huilin Yu 1 , Longhao Su 1 , Wantong Jia 1 , Mengjiao Jia 1 , Hongyu Pan 1 , Xianghui Zhang 1
Affiliation
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Chitosan, as a natural nontoxic biomaterial, has been demonstrated to inhibit fungal growth and enhance plant defense against pathogen infection. However, the antifungal pattern and mechanism of how chitosan application evokes plant defense are poorly elucidated. Herein, we provide evidence that chitosan exposure is fungicidal to C. heterostrophus. Chitosan application impairs conidia germination and appressorium formation of C. heterostrophus and has a pronounced effect on reactive oxygen species production, thereby preventing infection in maize. In addition, the toxicity of chitosan to C. heterostrophus requires Mkk1 and Mps1, two key components in the cell wall integrity pathway. The Δmkk1 and Δmps1 mutants were more tolerant to chitosan than the wild-type. To dissect chitosan-mediated plant defense response to C. heterostrophus, we conducted a metabolomic analysis, and several antifungal compounds were upregulated in maize upon chitosan treatment. Taken together, our findings provide a comprehensive understanding of the mechanism of chitosan-alleviated infection of C. heterostrophus, which would promote the application of chitosan in plant protection in agriculture.
中文翻译：
壳聚糖抑制异旋旋孢菌致病性的分子机制
壳聚糖作为一种天然无毒生物材料，已被证明可以抑制真菌生长并增强植物对病原体感染的防御能力。然而，壳聚糖的抗真菌模式和如何激发植物防御的机制尚不清楚。在此，我们提供证据表明暴露于壳聚糖对异旋梭菌具有杀菌作用。施用壳聚糖会损害异旋梭菌的分生孢子萌发和附着胞形成，并对活性氧的产生具有显着影响，从而预防玉米感染。此外，壳聚糖对异旋梭菌的毒性需要细胞壁完整性途径中的两个关键成分 Mkk1 和 Mps1。 Δ mkk1和 Δ mps1突变体比野生型对壳聚糖具有更强的耐受性。为了剖析壳聚糖介导的植物对异旋球菌的防御反应，我们进行了代谢组学分析，发现壳聚糖处理后玉米中的几种抗真菌化合物上调。综上所述，我们的研究结果提供了对壳聚糖减轻异旋梭菌感染的机制的全面了解，这将促进壳聚糖在农业植物保护中的应用。
更新日期：2024-02-16
中文翻译：
壳聚糖抑制异旋旋孢菌致病性的分子机制
壳聚糖作为一种天然无毒生物材料，已被证明可以抑制真菌生长并增强植物对病原体感染的防御能力。然而，壳聚糖的抗真菌模式和如何激发植物防御的机制尚不清楚。在此，我们提供证据表明暴露于壳聚糖对异旋梭菌具有杀菌作用。施用壳聚糖会损害异旋梭菌的分生孢子萌发和附着胞形成，并对活性氧的产生具有显着影响，从而预防玉米感染。此外，壳聚糖对异旋梭菌的毒性需要细胞壁完整性途径中的两个关键成分 Mkk1 和 Mps1。 Δ mkk1和 Δ mps1突变体比野生型对壳聚糖具有更强的耐受性。为了剖析壳聚糖介导的植物对异旋球菌的防御反应，我们进行了代谢组学分析，发现壳聚糖处理后玉米中的几种抗真菌化合物上调。综上所述，我们的研究结果提供了对壳聚糖减轻异旋梭菌感染的机制的全面了解，这将促进壳聚糖在农业植物保护中的应用。
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